Enzyme and DNA coding therefor
AssigneeJapat Ltd.
InventorsYasuno Iwasaki, Yoshiki Nishikawa
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Abstract
The invention concerns a peptidylhydroxyglycine N-C lyase (PHL) catalyzing the reaction R-GlyOH.fwdarw.R-NH.sub.2 wherein R represents a peptide residue, and GlyOH represents an .alpha.-hydroxyglycine residue linked to the C-terminus of said peptide R by an amide bond, a recombinant DNA molecule coding for a PHL, a method for the preparation of such a recombinant DNA molecule, processes for the preparation of PHL from a natural source or by means of the said recombinant DNA molecule, and the use of PHL for the preparation of an amidated peptide R-NH.sub.2.
Claims11
A recombinant DNA molecule selected from the group of molecules consisting of hybrid vectors and manufactured DNA molecules which can be used for preparation of hybrid vectors, said recombinant DNA molecule comprising a DNA sequence encoding the polypeptide from Xenopus laevis with peptidylhydroxyglycine N-C lyase (PHL) activity which polypeptide catalyzes the following reaction
- R-GlyOH.fwdarw.R--NH.sub.2
- wherein R represents a peptide residue, and GlyOH represents an .alpha.-hydroxyglycine residue linked to the C-terminus of said peptide R by an amide bond, said DNA sequence consisting essentially of the nucleotide sequence extending from position 1177 to 2148 of the sequence with SEQ ID No. 1.
Dependencies are inferred from the claim text.