Tissue fixative
AssigneeStreck Laboratories, Inc.
InventorWayne L. Ryan
Life of this patent
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Abstract
Tissue fixatives, such as diazolidinyl urea, which are free of aldehydes and toxic chemicals are described. When used, either in aqueous or alcoholic solutions, good tissue preservation is attained. In addition, tissue antigens are retained which makes the fixative useful for immunostaining procedures.
Claims34
A fixative solution for tissues and cells comprising histological fixing amounts of an active agent selected from the group consisting of:
- i) diazolidinyl urea
- ii) imidazolidinyl urea
- iii) dimethylol-5,5-dimethylhydantoin
- (iv) dimethylol urea
- (v) 2-bromo-2-nitropropane-1,3-diol;
- and
- (vi) quaternary adamantane
- in a solvent selected from the group consisting of water, alcohol, dimethylsulfoxide and mixtures thereof, said active agent being present in an amount of about 20 to 100 grams per liter.
In a method of fixing tissues or cells by treating same with a histological fixative, the improvement comprising employing as said histological fixative a composition comprising at least one of an active agent selected from the group consisting of:
- i) diazolidinyl urea
- ii) imidazolidinyl urea
- iii) dimethylol-5,5-dimethylhydantoin
- iv) dimethylol urea
- v) 2-bromo-2-nitropropane-1,3-diol;
- and
- vi) quaternary adamantane
- in a solvent selected from water, an alcohol and mixtures thereof, said active agent being present in an amount of about 20 to 100 grams per liter.
Dependencies are inferred from the claim text.