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US 5,194,383 AGranted
Process for making L-aminoacylase
AssigneeNational Science Council of Republic of China
InventorsTsai, Ying C., Hsiang L. Hu, Yunn B. Yang
Life of this patent
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Abstract
A process for making L-aminoacylase includes a cultivation of microorganism selected from a specy of Alcaligenes, especially the Alcaligenes denitrificans DA 181, and a separation of a produced L-aminoacylase from the bacterial cells for obtaining the L-aminoacylase which may be further purified for the production of L-amino acid. The acylase made by such a process may have an increased stability, beneficial for its commercial and medical values.
Claims3
A process for making L-aminoacylase comprising:
- culturing Alcaligenes denitrificans DA181 in a culturing medium for growing cells for forming L-aminoacylase enzyme;
- and isolating said L-aminoacylase enzyme from the cells of the culturing medium.
A process for making L-aminoacylase comprising:
- culturing Alcaligenes denitrificans strain DA181 in a culturing medium by a liquid cultivation method for growing cells for forming an enzyme of L-aminoacylase, and isolating, and purifying said L-aminoacylase from said cells and said culturing medium, said L-aminoacylase having a plurality of properties including:
- a substrate specificity capable of hydrolyzing N-acyl-L-amino acids and unable for hydrolyzing N-acyl-D-amino acids, having a molecular weight of 80,000 measured by gel filtration method and 40,000 by SDS-PAGE method, having an isoelectric point of 5.1, a thermal stability of 80% of remaining activity at 60.degree. C. for one hour and a pH stability at pH value of 5-9, an optimum reaction temperature of 60.degree.-70.degree. C. and optimum reaction pH value of 8.0, and being inhibited by lead, zinc, ferrous, cupric, and mecuric ions.
Dependencies are inferred from the claim text.