Cephalosporin C acylase
AssigneeFujisawa Pharmaceutical Co., Ltd.
InventorsMorita Iwami, Ichiro Aramori, Masao Fukagawa, Takao Isogai, Hitoshi Kojo
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Abstract
A cephalosporin C acylase from Pseudomonas diminuta N-176 is disclosed as well as the recombinant production of this enzyme in E. coli. The enzyme is characterized by the ability to catalyze the conversion of cephalosporin C, gultaryl 7-ACA, apidyl 7-ACA, succinyl 7-ACA, N-acetylcephalosporin C, N-benzoylcephalosporin C, and cephalothin into 7-aminocephalosporanic acid and is composed of an .alpha.-subunit with a molecular weight of 26,000 daltons and a .beta.-subunit with a molecular weight of 58,000 daltons.
Claims2
An isolated and purified cephalosporin C acylase having the following characteristics:
- (a) has ability to catalyze the enzymatic conversion of cephalosporin C, glutaryl 7-ACA, adipyl 7-ACA, succinyl 7-ACA, N-acetylcephalosporin C, N-benzoylcephalosporin C and cephalothin into 7-amino cephalosporanic acid,
- (b) is composed of an .alpha.-subunit with a molecular weight of 26,000 daltons as measured by SDS-PAGE and a .beta.-subunit with a molecular weight of 58,000 daltons as measured by SDS-PAGE,
- (c) has N-terminal amino acid sequence of the .alpha.-subunit:
- Thr-Met-Ala-Ala-Asn-Thr-Asp-Arg-Ala-Val-Leu-Gln-Ala-Ala-Leu-Pro-Pro-Leu- (SEQ ID NO:1).
Dependencies are inferred from the claim text.