Purification of LFA-3
AssigneeDana-Farber Cancer Institute, Inc.
InventorsMichael Dustin, Timothy Springer
Life of this patent
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Abstract
A method of purifying LFA-3 using affinity chromatography. Purified LFA-3 is useful for quantitating or separating out CD-2-containing cells.
Claims12
A method for purifying LFA-3comprising contacting a liquid comprising LFA-3 with an affinity column comprising anti-LFA-3 antibody to bind LFA-3 to said anti-LFA-3 antibody, washing said column with a first alkaline buffer, and then eluting said LFA-3 from said affinity column using a second acidic buffer, to provide purified LFA-3 capable of binding to CD2 antigen on T-lymphocytes.
A method of detecting a cell bearing a CD2 antigen, said method comprising detecting binding of purified LFA-3 to a cell, said LFA-3 being capable of binding to CD2 antigen on T-lymphocytes, wherein said binding specifically indicates the presence of CD2 antigen on said cell.
A method of specifically separating cells bearing CD2 antigen from other cells in a mixture, said method comprising contacting a mixture of cells including CD2 antigen-bearing cells with a solid support comprising purified LFA-3 capable of binding to CD2 antigen on T-lymphocytes, to bind said CD2-antigen-bearing cells thereto.
Substantially pure LFA-3 capable of binding to CD2 antigen on T-lymphocytes.
Dependencies are inferred from the claim text.