Displacement immunoassay utilizing an oligavalent labelled antibody
AssigneeBoehringer Mannheim GmbH
InventorsDieter Mangold, Reiner Schlipfenbacher
Life of this patent
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Abstract
A method for carrying out competitive displacement immunoassays which entails the use of an oligovalent labelled antibody having at least 4 binding sites for the analyte to be determined per label.
Claims9
Method for determining an analyte in a liquid sample, comprising contacting said liquid sample to an immobilized complex of (i) immobilized analyte or analyte analogue and (ii) an obligovalent labelled antibody which binds to said analyte to be determined, said oligovlent labelled antibody produced by conjugating antibodies having 2 or fewer binding sites and characterized by at least 4 binding sites for said analyte or analyte analogue per label, under conditions favoring displacement of said obligovalent labelled antibody from said immobilized analyte or analyte analogue and formation of soluble complexes of analyte to be determined and oligovalent labelled antibody, and determining oligovalent labelled antibody bound to said immobilized analyte or analyte analogue or in said liquid sample as a determination of analyte to be determined.
Dependencies are inferred from the claim text.