DNA sequences encoding BMP-6 proteins
AssigneeGenetics Institute, Inc.
InventorsJohn M. Wozney, Elizabeth A. Wang, Vicki A. Rosen, Anthony J. Celeste
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Abstract
Purified BMP-6 proteins and processes for producing them are disclosed. The proteins may be used in the treatment of bone and/or cartilage defects and in wound healing and related tissue repair.
Claims27
An isolated DNA sequence encoding a BMP-6 protein said DNA sequence comprising the nucleotide sequence selected from the group consisting of:
- a) nucleotide #1 through nucleotide #666 of FIG. 2;
- b) nucleotide #1393 to #1698 of FIG. 3;
- c) nucleotide #1 to #2393 of FIG. 3;
- d) nucleotide #160 to #1698 of FIG. 3;
- e) nucleotide #1303 to #1698 FIG. 3;
- f) allelic variants and degenerative sequences of (a) through (d);
- and g) sequences which
- (1) hybridize to said DNA sequence under stringent hybridization conditions;
- and
- (2) encode a protein characterized by the ability to induce the formation of cartilage and/or bone in the Rose-modified Sampath-Reddi assay.
An isolated DNA sequence encoding the BMP-6 protein of FIG. 3 or a fragment thereof which encodes a protein characterized by the ability to induce the formation of cartilage and/or bone in the Rosen-modified Sampath-Reddi assay" after "3".
An isolated DNA sequence encoding a BMP-6 protein said sequence comprising nucleotide #1 to #2923 of FIG. 3.
A method for producing a purified BMP-6 protein said method comprising the steps of
- (a) culturing in a suitable culture medium cells transformed with a DNA sequence comprising the DNA nucleotide #1 to #2923 of FIG. 3;
- and
- (b) isolating and purifying said protein from said culture medium said BMP-6 protein.
An isolated DNA sequence encoding a BMP-6 protein said sequence comprising nucleotide #160 to #1698 of FIG. 3.
A method for producing a purified BMP-6 protein said method comprising the steps of
- (a) culturing in a suitable culture medium cells transformed with a DNA sequence comprising the DNA nucleotide #5/8to #1698 of FIG. 3;
- and
- (b) isolating and purifying said protein from said culture medium said BMP-6 protein.
An isolated DNA sequence encoding a BMP-6 protein said sequence comprising nucleotide #1303 to #1698 of FIG. 3.
A method for producing a purified BMP-6 protein said method comprising the steps of
- (a) culturing in a suitable culture medium cells transformed with a DNA sequence comprising the DNA nucleotide #1303 to #1698 of FIG. 3;
- and
- (b) isolating and purifying said protein from said culture medium said BMP-6 protein.
An isolated DNA sequence encoding a BMP-6 protein said sequence comprising nucleotide #1393 to #1698 of FIG. 3.
A method for producing a purified BMP-6 protein said method comprising the steps of
- (a) culturing in a suitable culture medium cells transformed with a DNA sequence comprising the DNA nucleotide #1393 to #1698 of FIG. 3;
- and
- (b) isolating and purifying said protein form said culture medium said protein comprising amino acid #412 to #513 as shown in FIG. 3.
An isolated DNA sequence encoding the BMP-6 protein of FIG. 2.
An isolated DNA sequence encoding a BMP-6 protein comprising the nucleotide sequence from nucleotide #1 to #666 of FIG. 2.
Dependencies are inferred from the claim text.