cDNA clone coding for Venezuelan equine encephalitis virus and attenuating mutations thereof
AssigneeNorth Carolina State University
InventorsNancy L. Davis, Loretta V. Willis, Robert E. Johnston, Jonathan F. Smith
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Abstract
A DNA comprises a cDNA clone coding for an infectious Venezuelan Equine Encephalitis Virus RNA transcript and a heterologous promoter sequence positioned upstream from the cDNA clone and operatively associated therewith. A method of making a live attenuated Togavirus useful as a vaccine, and cDNA clones which code for attenuated Togaviruses, is also disclosed.
Claims5
A DNA comprising a cDNA clone coding for an infectious Venezuelan Equine Encephalitis (VEE) Virus RNA transcript and a heterologous promoter positioned upstream from said cDNA clone and operatively associated therewith, and further comprising at least one attenuating mutation selected from the group consisting of codons at E2 amino acid position 76 which specify an attenuating amino acid and codons at E2 amino acid position 209 which specify an attenuating amino acid.
Dependencies are inferred from the claim text.