Method of making calibration solution for verifying calibration and linearity of vertical photometers
OwnerMary J. Freeman, Gary M. Freemaninventors
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Abstract
A method and device for calibrating or checking the calibration of vertical photometers. The invention may also provide easy and convenient assay results for diagnostic profiles. The invention comprises pre-dispensed amounts of a suitable reagent, chosen for the particular use, disposed in a vessel with an amount of a wetting agent to yield a reproducible meniscus in the resulting control solution. The control solution is dried to leave a precise amount of dried reagent and other constituents in the vessel which can then be stored and distributed until subsequent use. The dried control solution can then be reconstituted to provide a standard having known absorbance and physical characteristics.
Claims9
A method of making a test kit to be used for taking vertical photometric measurements comprising:
- (1) providing a control solution having at least one reagent and a wetting agent therein, provided in at least one vessel, said control solution having predetermined and predictable physical characteristics, said wetting agent forming a predetermined meniscus within said vessel,
- (2) drying said solution to obtain a dried residue within said vessel having a pre-dispensed amount of said reagent, wherein said dried residue may be stored for subsequent use,
- (3) reconstituting said dried residue using an amount of an activator to reform said control solution such that said reformed control solution will have substantially equivalent physical characteristics to said control solution provided in step one, including substantially reforming said predetermined meniscus in said vessel, such that said reformed control solution may be used to obtain accurate measurements based upon the pre-dispensed amount of said reagent.
A method of making an apparatus for calibration of and determination of the linearity of a vertical photometer comprising the steps of:
- providing a stable liquid dye solution having a known absorbance value and predetermined physical characteristics, said solution including an amount of a wetting agent,
- placing predetermined amounts of said dye solution in separate vessels of predetermined volume, said wetting agent in said solution acting to produce a predetermined meniscus within said vessels,
- drying said amount of said liquid dye solution to form dehydrated dyes in said vessels which may be reconstituted utilizing an activator liquid so as to have substantially similar physical characteristics including said predetermined meniscus within said vessels and known absorbance values such that measurement of the absorbance values of said reconstituted dye solutions using a vertical photometer will give an indication of calibration of the vertical photometer and measurement of the absorbance values of said separate vessels will give an indication of the linearity of said vertical photometer.
Dependencies are inferred from the claim text.