Method for production of human tissue type plasminogen activator
AssigneeMitsui Toatsu Chemicals, Inc.
InventorsShoichiro Miyahara, Atsunori Shindo, Maki Suzuki, Nobumi Kusuhara, Nobuyoshi Makiguchi
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Abstract
A method for the production of human tissue type plasminogen activator (human t-PA) using cells is disclosed. The method comprises producing the human t-PA in parallel with the growth of the cells or after the cells have been grown. To the cell culture bicarbonate ion is added at a concentration equivalent to 3 to 10 g/l of NaHCO.sub.3 based on the true volume of the medium to increase the osmotic pressure of the medium to a range of 350 to 520 milliosmoles/liter.
Claims4
A method for producing human tissue type plasminogen activator using cells, wherein the human tissue type plasminogen activator is produced in parallel with growth of the cells in a cell culture medium in which bicarbonate ion equivalent to 3 to 10 g/l of NaHCO.sub.3 based on the true volume of the medium is supplied to said cell culture medium to increase the osmotic pressure of the medium to a range of 350 to 520 milliosmoles/liter.
A method for producing human tissue type plasminogen activator using cells, wherein the cells are grown in advance in a medium and then incubated in a medium for the production of human tissue type plasminogen activator, the osmotic pressure in said medium for production of human tissue type plasminogen activator being increased using bicarbonate ion equivalent to 3 to 10 g/l of NaHCO.sub.3 based on the true volume of the medium is supplied to said medium to increase the osmotic pressure of the medium to a range of 350 to 520 milliosmoles/liter.
Dependencies are inferred from the claim text.