Hantaan virus strain ROK84/105 and vaccine therefor
AssigneeKorea Green Cross Corporation
InventorsHo W. Lee, Sun J. Riu, Chang N. An
Life of this patent
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Abstract
The present invention relates to Hantaan virus and a vaccine therefor. The Hantaan virus vaccine can be prepared as follows: The Hantaan virus ROK84/105 strain isolated from the human body as a seed strain, injected to one-day-old suckling mouse or rat, which is raised for about 10 days. Thereafter, the head of the mouse or rat is cut apart after paralyzing the mouse or rat to take the brain, the brain is treated with ethyl alcohol and protamine sulfate solution, the resultant is purified and inactivated, and an adjuvant is added to the final product.
Claims15
A vaccine for Hantaan virus which is prepared by a process comprising the steps of introducing Hantaan virus strain ROK 84/105 into a rat or mouse, subsequently removing brain tissue from the brain of the rat or mouse, extracting the brain tissue with an extraction fluid, purifiying the resulting virus extract, and inactivating the virus in the resulting virus extract, wherein Hantaan virus strain ROK 84/105 is a strain of Hantaan virus having an international deposit number of ATCC VR 2250.
A viral vaccine comprising a plurality of virion particles, said plurality of virion particles being Hantaan virus particles of the strain ROK 84/105 and having an International Deposit Number of ATCC VR 2250.
A viral vaccine comprising a plurality of virion particles, said plurality of virion particles being Hantaan virus particles of the strain ROK 84/105 having an international deposit number of ATCC VR 2250, wherein each of said plurality of Hantaan virus virion particles include:
- a single strand of RNA having a plurality of definable segments, said RNA strand including at least one negative-sense RNA genome, each of said plurality of virion particles having a spherical or an ellipsoid shape having a diameter of about 95 nm, a lipoprotein envelope enclosing a plurality of nucleocapsids, and a plurality of fibers extending outwardly from said envelope a distance of about 5-10 nm, and wherein the titer of the virus in Vero E6 cells is 10.sup.7.2 /ml.
Dependencies are inferred from the claim text.