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US 5,182,215 AGranted
Screening test for pineocytoma
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsDavid C. Klein, Horst W. Korf, Jeffrey N. Bruce
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Abstract
A novel method of diagnosing pineal cell tumors by detecting S-antigen in CSF is described.
Claims2
A screening test for pineocytoma, comprising the steps of:
- a) obtaining cerebrospinal fluid from an individual;
- b) contacting said fluid with an antibody which specifically binds S-antigen, under conditions effective for and a time sufficient for binding of said antibody to said S-antigen to occur;
- c) detecting any S-antigen from said fluid bound to said antibody, the presence of S-antigen in said fluid being indicative of pineocytoma in the individual from whom said cerebrospinal fluid was obtained.
A screening test for pineocytoma, comprising the steps of:
- a) obtaining cerebrospinal fluid from an individual;
- b) separating said fluid into components by electrophoresis on a polyacrylamide gel;
- c) transferring said components onto a nitrocellulose filter;
- d) contacting said filter with an antibody which specifically binds S-antigen, under conditions effective for and a time sufficient for binding of said antibody to said S-antigen to occur;
- e) detecting any S-antigen from said filter bound to said antibody, the presence of S-antigen on said filter being indicative of pineocytoma in the individual from whom said cerebrospinal fluid was obtained.
2 more elements
Dependencies are inferred from the claim text.