Method for detecting the presence of peroxidatively active substance in basic media
AssigneeMiles Inc.
InventorsMarvin A. Genshaw, Michael J. Pugia
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Abstract
An assay for the detection of a peroxidatively active substance in a test sample. The assay employs a phenylenediamine and a naphthol which, under the conditions of the assay, couple to form a chromogen thereby indicating the presence of the analyte. The assay is carried out at a pH of from 10 to 14 since false positives due to ascorbate interference are minimized in highly alkaline media.
Claims7
An assay for the detection of a peroxidatively active substance in an aqueous test sample which comprises providing an aqueous medium having a pH of 10 to 14 suspected of containing the peroxidatively active substance to be detected and adding to such medium a peroxide together with a phenylenediamine and a naphthol which, under the conditions of the assay, couple to form a chromogen thereby providing a detectable color change in the medium in the presence of the peroxidatively active substance.
An assay for the detection of hemoglobin in an aqueous test sample having a pH of from 10 to 14 which comprises the steps of adding to an aqueous medium suspected of containing hemoglobin, a peroxide together with a phenylenediamine and a naphthol which, under the conditions of the assay, couple to form a chromogen when hemoglobin is present thereby providing a detectable color change in the medium.
Dependencies are inferred from the claim text.