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US 5,182,203 AGranted
Bifunctional compounds useful in catalyzed reporter deposition
AssigneeE. I. Du Pont de Nemours and Company
InventorsRichard C. Ebersole, John R. Moran
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Abstract
Novel bifunctional hydroxyphenylazobenzoic acid analogues (HABA-type and conjugates) and biotin analogues probiotin-type conjugates) useful as reagents in assays employing catalyzed reporter deposition are described as well as intermediates useful in synthesizing these compounds.
Claims2
A compound of the formula:
- ##STR10## wherein:
- R.sup.1 through R.sup.8 are the same or different and are selected from the group consisting of H, OH, OCH.sub.3, straight chain or branched alkyl groups having 1-4 carbon atoms, F, Cl, Br and;
- Z.sup.1 and Z.sup.2 are independently phenyl or napthyl;
- n is 1-19;
- X is N, O, S;
- R.sup.9 is selected from the group consisting of glycosides and straight chain or branched alkyl groups having 1-4 carbon atoms;
- L is (NH(CH.sub.2).sub.s CO).sub.r where s is 1-5 and r is 0-5;
- and
- R is a reporter selected from the group consisting of radioisotopes, fluorogenic, light scattering, chemiluminescent, electrochemical and magnetic materials and enzymes provided that the enzyme does not react with OR.sup.9.
6 more elements
A compound which is 6-(phenoxy-(4'-azo-2"-carboxyethylphenyl)-hexanoyl-alkaline phosphatase.
Dependencies are inferred from the claim text.