Lipase immobilization without covalent bonding on an amphiphilic support containing lipophilic alkyl chains
AssigneeUop LLC
InventorYoshihisa Tsuda
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Abstract
A support matrix particularly suitable for the immobilization of biologically active materials acting on lipophilic substrates results from the reaction of a polyamine-impregnated porous particle with a monofunctional paraffinic aldehyde. The resulting support matrix has a hydrophobic coating providing a microenvironment for the biologically active material, such as an enzyme, and hydrophilic portions for emulsification or binding to polar regions at or near the active site.
Claims33
A method of making an amphiphilic support matrix for the immobilization of lipophilic biologically active materials without covalent bonding comprising impregnating a hard, incompressible porous particle with a hydrophilic polyamine, to provide a coating of hydrophilic polyamine on the particle reacting the hydrophilic polyamine coating with a monofunctional, saturated aliphatic aldehyde containing a lipophilic alkyl chain of at least 5 carbon atoms, to convert the hydrophilic polyamine coating to a hydrophobic coating while retaining hydrophilic sites to produce an amphiphilic support matrix removing unreacted aldehyde, and recovering the resulting amphiphilic support matrix.
A method of making an immobilized lipase comprising impregnating a hard, incompressible porous particle with a hydrophilic polyamine to provide a coating of hydrophilic polyamine on the particle, reacting the hydrophilic polyamine coating with a monofunctional, saturated aliphatic aldehyde containing a lipophilic alkyl chain of at least 5 carbon atoms to convert the hydrophilic polyamine coating to a hydrophobic coating while retaining hydrophilic sites to produce an amphiphilic support matrix;
- removing unreacted aldehyde, contacting the resulting amphiphilic support matrix with an aqueous solution of a lipase under immobilizing conditions for a time effective to immobilize the lipase thereon without covalent bonding, and recovering the resulting immobilized lipase.
Dependencies are inferred from the claim text.