Expression systems for overproduction of desired proteins
AssigneeBiogen, Inc.
InventorsBernard Allet, Eric H. Kawashima
Life of this patent
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Abstract
Recombinant DNA molecules comprising DNA sequences derived from bacteriophage T4 that are useful in expressing desired polypeptides in unexpectedly high yields, hosts and expression systems comprising such recombinant DNA molecules and methods for expressing desired polypeptides in high yields by the utilization of such hosts and expression systems.
Claims13
A method for improving the expression of a non-bacteriophage T4 protein comprising culturing a prokaryotic host transformed with a recombinant DNA molecular capable of directing expression of said non-bacteriophase T4 protein in high yields in said prokaryotic host, said recombinant DNA molecule comprising the following elements, operatively linked in the following order, 5' to 3':
- DNA sequence I, DNA sequence X, DNA sequence coding for a non-bacteriophage T4 protein, wherein said DNA sequence I consists of:
- ##STR9## said DNA sequence X comprises a DNA sequence selected from the group consisting of CGATACT, CGCGATACT, ATACTAAA, ATACT, OGCGATACTAAA, CGATACTAA and CGATTCC and said non-bacteriophase T4 protein is selected from the group consisting of human interleukin 2 and human tumor necrosis factor.
A recombinant DNA molecule comprising the following elements, operatively linked in the following order, 5' to 3':
- DNA sequence I, DNA sequence X, DNA sequence coding for a non-bacteriophage T4 protein, wherein said DNA sequence I consists of:
- ##STR11## said DNA sequence X comprises a DNA sequence selected from the group consisting of CGATACT, CGCGATACT, ATACTAAA, ATACT, CGCGATACTAA, CGATACTAAA and CGATTCC and said non-bacteriophage T4 protein is selected from the group consisting of human interleukin 2 and human tumor necrosis factor, said recombinant DNA molecule directing expression of said non-bacteriophage T4 protein in high yields in a prokaryotic host.
Dependencies are inferred from the claim text.