Method for increasing gene expression using protease deficient yeasts
AssigneeTakeda Chemical Industries, Ltd.
InventorsKazuo Nakahama, Yoshihiko Kaisho, Koji Yoshimura
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Abstract
Disclosed are respiratory-deficient yeast except Saccharomyces cerevisiae AH22R.sup.-, said respiratory-deficient yeast being transformed with a DNA containing a gene encoding a protein foreign to yeast and a method for preparing a protein foreign to yeast described above, which comprises culturing the yeast, and producing and accumulating the protein in a culture. The respiratory-deficient yeast of the present invention can produce a higher amount of protein than its parent strain.
Claims7
Respiratory-deficient yeast excluding Saccharomyces cerevisiae AH22R.sup.-, said respiratory-deficient yeast being transformed with a DNA containing a gene encoding a protein foreign to yeast and capable of expressing a foreign DNA, wherein the yeast is Saccharomyces cerevisiae NA74-3A(.rho..sup.-), Saccharomyces cerevisiae K33-7B(.rho..sup.-), Saccharomyces cerevisiae NAX-50D(.rho..sup.-), Saccharomyces cerevisiae NA74-3A(.rho..sup.-)/pTFL710T, Saccharomyces cerevisiae NA74-3A(.rho..sup.-)/pPFL725T, Saccharomyces cerevisiae NA74-3A(.rho..sup.-)/pGFE313, or Saccharomyces cerevisiae NAX-50D(.rho..sup.-)/pTFL710T.
Dependencies are inferred from the claim text.