Transformation of plants to introduce closely linked markers
AssigneeDNA Plant Technology Corporation
InventorRichard A. Jorgensen
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Abstract
A novel method of producing a plant with a marker closely linked to a target locus, in particular a nuclear male sterile target locus, is described. The method involves transformation of a group of plants in order to introduce a marker into each plant, and isolation of a plant with the marker closely linked to a target locus. The markers include visible markers and dominant conditional lethal markers. The method is of particular use for hybrid seed production where the target locus is a nuclear male sterile locus.
Claims40
A method of producing a close-linkage dicotyledonous plant containing a marker closely linked to a male sterile locus, comprising:
- a. transforming a first dicotyledonous plant with a marker foreign to the species of said first dicotyledonous plant to produce a group of transformants, said marker being substantially randomly inserted into the genome of the plant and said transforming being carried out by use of Agrobacterium;
- b. selecting a group of single locus transformants from the group of transformants;
- and
- c. selecting from the group of single locus transformants a close-linkage plant containing a marker in close linkage with a male sterile locus, said close linkage being linkage of less than about 10 recombination units.
A method of producing hybrid seed in a dicotyledonous plant, comprising:
- a. transforming a first dicotyledonous plant with a marker foreign to the species of said first dicotyledonous plant to produce a group of transformants, said marker being substantially randomly inserted into the genome of the plant and said transforming being carried out by use of Agrobacterium;
- b. selecting a group of single locus transformants from the group of transformants;
- c. selecting from the group of single locus transformants a close-linkage plant containing a marker in close linkage with a male sterile locus, said close linkage being linkage of less than about 10 recombination units;
- d. crossing the close-linkage plant and selecting a close-linkage maintainer line heterozygous for male fertility;
- e. crossing the close-linkage maintainer line with a homozygous male sterile line to yield a mixture of male fertile and male sterile plants;
- f. removing male fertile plants from the mixture based on the presence of the marker to leave a group of plants at least 90% of which are male sterile;
- and
- g. pollinating said group of plants at least 90% of which are male sterile to yield hybrid seed.
A close-linkage, dicotyledenous maintainer plant heterozygous for male fertility, said maintainer plant comprising a dominant marker foreign to the species of said maintainer plant closely linked in coupling to a male sterile locus at a linkage of less than about 10 recombination units.
Dependencies are inferred from the claim text.