Chromophoric reagents for incorporation of biotin or other haptens into macromolecules

Abstract

Compounds and methods for detecting the incorporation of a hapten into a protein or macromolecules are disclosed. The compounds comprise haptens bound directly or indirectly to a chromophoric group and a macromolecules-reactive group, whereby incorporation of the hapten into a protein or other macromolecules may be detected. The disclosure includes a compound of the formula: ##STR1## wherein n.sub.1 is 1, 2, 3, 4, 5, 6 or 7; n.sub.2 is 0, 1, 2, 3, 4, 5, 6 or 7; R.sub.1 is either a carboxytetramethyl-rhodamyl, sulforhodamyl 101, or dinitrophenylaminohexanoyl group; and R.sub.2 is --CO.sub.2 H, --NH.sub.2, --SH, ##STR2##

Dependencies are inferred from the claim text.