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US 5,180,818 AGranted
Site specific cleavage of single-stranded DNA
AssigneeUniversity of Colorado Foundation, Inc.
InventorsThomas R. Cech, Daniel Herschlag
Life of this patent
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Abstract
Method for specifically cleaving a single stranded DNA molecule. The method includes providing an RNA molecule having a deoxyribonuclease activity independent of any protein, and contacting that RNA molecule with the single stranded DNA molecule to cause the single stranded DNA molecule to be cleaved.
Claims8
A method for specifically cleaving a single-stranded DNA molecule, comprising the steps of:
- (a) providing a first RNA molecule selected from the group consisting of a group I intron, a group II intron, or RNAse P, that cleaves a second RNA molecule to leave a 3'-OH, said first RNA molecule having a deoxyribonuclease activity, and
- (b) contacting said first RNA molecule with said single-stranded DNA molecule under conditions which allow said first RNA molecule to cause said single-stranded DNA molecule to be cleaved said conditions including providing Mg.sup.2+ ions and guanosine or guanosine triphosphate at a pH between 6.0 and 9.5 and a temperature between 20.degree. C. and 70.degree. C.
Dependencies are inferred from the claim text.