Lysing reagent for leukocyte differentiation method
AssigneeFisher Scientific Company
InventorsLudmilla P. Di Ianni, Edward L. Carver, Jr.
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Abstract
A lysing reagent is employed for stromatolysing the red cells in a blood sample and differentiating the leukocytes into subpopulations. The lysing reagent contains a dodecyltrimethylammonium halide and an aromatic second cationic agent which is either a benzyltrialkylammonium halide or an alkylpyridinium halide. In some methods, the lysing reagent is mixed in the reaction chamber with a major proportion of the diluent, and the blood sample with a minor proportion of the diluent is added to the reaction chamber.
Claims25
In a lysing reagent for use in automated hematological measurement being an aqueous solin containing dodecyltrimethyl ammonium halide and a second cationic agent, the improvement wherein the second cationic agent is selected from the group consisting of benzyltrialkylammonium halides of the formula:
- Ph--CH .sub.2 -N.sup.+ (R).sub.2 -Q X.sup.-
- and alkylpyridinium halides of the formula:
- A.dbd.N.sup.+ Q x.sup.-
- wherein Ph is phenyl, R is methyl or ethyl, Q is alkyl of 14 to 16 carbons, A=N is pyridinium and X is chlorine or bromine.
In a method for stromatolyzing a blood sample by mixing with a solution of cationic agents to produce a reaction mixture in which the leukocytes have been differentiated by size into subpopulations, with one of the cationic agents being a dodecyltrimethylammonium halide, the improvement wherein another of the cationic agents is selected from the group consisting of benzyltrialkylammonium halides of the formula:
- Ph--CH.sub.2 -N.sup.+ (R).sub.2 -Q X.sup.-
- and alkylpyridinium halides of the formula:
- A.dbd.N.sup.+ Q X.sup.-
- wherein Ph is phenyl, R is methyl or ethyl, Q is alkyl of 14 to 16 carbons, A=N is pyridinium and X is chlorine or bromine.
Dependencies are inferred from the claim text.