Saccharide compositions, methods and apparatus for their synthesis
AssigneeThe Trustees of the University of Pennsylvania
InventorStephen Roth
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Abstract
A method and an apparatus for preparing saccharide compositions is disclosed. The m GOVERNMENT SUPPORT Portions of this invention were supported by National Science Foundation Grant DCB8817883.
Claims25
An apparatus for a glycosyltransferase-catalyzed synthesis of a saccharide composition from saccharide units and an acceptor moiety, said apparatus comprising:
- a reactor;
- at least four different glycosyltransferases specific for the synthesis of said saccharide composition in said reactor;
- inlet means for introducing an acceptor moiety and a plurality of required saccharide units into said reactor such that said saccharide composition is synthesized;
- and
- outlet means for discharging said saccharide composition from said reactor;
- wherein said acceptor moiety is one member selected from the group consisting of proteins, glycoproteins, lipids, glycolipids and carbohydrates.
An apparatus for a glycosyltransferase-catalyzed synthesis of a saccharide composition from saccharide units and an acceptor moiety, said apparatus comprising:
- a reactor;
- at least three different glycosyltransferases specific for the synthesis of said saccharide composition in said reactor;
- inlet means for introducing an acceptor moiety and a plurality of required saccharide units into said reactor such that said saccharide composition is synthesized;
- and
- outlet means for discharging said saccharide composition from said reactor;
- wherein said acceptor moiety is one member selected from the group consisting of proteins, glycoproteins, lipids, glycolipids and carbohydrates.
An apparatus for a glycosyltransferase-catalyzed synthesis of a tetrasaccharide composition of formula:
- gal-glcNAc-gal-.beta.-1,4-glc, said apparatus comprising:
- a reactor;
- at least three different members selected from the group consisting of N-acetyl glucosaminyltransferases and galactosyltransferases suitable for the synthesis of said tetrasaccharide composition in said reactor;
- inlet means for introducing lactose, UDPGlcNAc and UGPgal into said reactor such that a tetrasaccharide composition of gal-glcNAc-gal-.beta.-1,4-glc is synthesized;
- and
- outlet means for discharging said tetrasaccharide composition from said reactor.
Dependencies are inferred from the claim text.