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US 5,180,672 AGranted
Ascorbate oxidase isolated from Acremonium sp. HI-25 ferm BP-3124
AssigneeAsahi Kasei Kogyo Kabushiki Kaisha
InventorsHomare Itoh, Takashi Shin, Takahisa Yajima, Shigeki Fukuyasu
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Abstract
An ascorbate oxidase having an optimum pH range of 3.5-4.5 is isolated from Acremonium sp. Hi-25 BP-3124. The ascorbate oxidase is used in foods or drinks to oxidize ascorbate to prevent damage to the foods or drinks by oxidation.
Claims1
An isolated ascorbate oxidase having the properties as mentioned below:
- (1) having a pH optimum in the range of pH 3.5-4.5;
- (2) being activated by Fe.sup.++ and inhibited by Cu.sup.++ ;
- (3) having a molecular weight of 85,000.+-.5,000 as determined by the gel filtration method, and subunit molecular weight of 23,000+2,000 as determined by the sodium dodecyl sulfate/polyacrylamide gel electrophoresis method;
- (4) having an isoelectric point of pI 4.0 as measured by the isoelectric focusing method;
- (5) showing stable activity at pH 8.0 when heated to a temperature as high as 60.degree. C. for 30 minutes;
- and
- (6) showing stable activity at 4.degree. C. over the pH range of pH 4-11.
4 more elements
Dependencies are inferred from the claim text.