Method for purification of mitomycin C

Abstract

A method for purification of mitomycin C comprises; a. a step of treating the culture of a mitomycin C-producing microorganism with reverse phase adsorption resin to adsorb mitomycin C onto the resin; b. a step of eluting mitomycin C with ethyl acetate out of the resin; c. a step of adding phosphate buffer to the eluate and then evaporating ethyl acetate; d. a step of passing, under pressure, the residue remaining after the evaporation through a column packed with a reverse phase adsorbent having a small particle diameter to adsorb mitomycin C onto the resin; e. a step of eluting mitomycin C with aqueous methanol out of the resin under pressure; f. a step of treating the eluate with a reverse phase adsorption resin to adsorb mitomycin C onto the resin; g. a step of eluting mitomycin C with methanol out of the resin; and, h. a step of concentrating the methanol eluate to crystallize mitomycin C. By this method, mitomycin C can be efficiently purified in an industrial scale.

Dependencies are inferred from the claim text.