Immunochemical assays for human amylase isoenzymes and related monoclonal antibodies, hydridoma cell lines and production thereof
AssigneeThe University of Virginia Alumni Patents Foundation
InventorsDavid Bruns, David Benjamin
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Abstract
Immunochemical assays for human amylase isoenzymes are disclosed. These assays utilize monoclonal antibodies which are produced from hybridoma cell lines and are specific for human salivary amylase and ovarian tumor amylase but not human pancreatic amylase. The assays involve reacting this monoclonal antibody with the unknown sample for the purpose of studying quantitatively and qualitatively the amylase isoenzymes present. The results from such assays are useful in the diagnosis of disease or ruling out disease. Antibody producing hybridoma cell lines and their production are described.
Claims19
A hybridoma cell line produced from a hybridoma designated ATCC HB-8984 which produces and secretes HABB monoclonal antibodies reactive with human salivary amylase and ovarian tumor amylase but not human pancreatic amylase.
A HABB monoclonal antibody which reacts with human salivary amylase and ovarian tumor amylase but not with human pancreatic amylase.
A process for identifying and measuring specific amylase isoenzymes using HAAB monoclonal antibodies comprising reacting the monoclonal antibodies reactive to human salivary amylase and ovarian tumor amylase but not human pancreatic amylase, with amylases in a biological sample, and measuring the pancreatic amylase.
A process of measuring pancreatic amylase comprising first determining the total amylase concentration in a part of a sample and then reacting another part of the same sample with HABB monoclonal antibodies which are reactive to human salivary amylase and ovarian tumor amylase but not human pancreatic amylase, which have also been coupled to an insoluble matrix, centrifuging the insoluble complex down to a pellet, taking the supernatant and determining the total amylase therein which is the pancreatic amylase.
A hybridoma having an ATCC designation of HB-8984.
HABB monoclonal antibodies produced by hybridoma cell line produced from a hybridoma designated ATCC HB-8984.
A hybridoma cell line produced from a hybridoma designated ATCC HB-8984 produced from an ovarian tumor amylase immunogen which produced and secretes monoclonal antibodies reactive with human salivary amylase and ovarian tumor amylase but not reactive with human pancreatic amylase.
Monoclonal antibodies produced from a hybridoma designated ATCC HB-8984 resulting from an ovarian immunogen which reacts with human salivary amylase and ovarian tumor amylase but not with human pancreatic amylase.
A process for identifying and measuring specific amylase isoenzymes using HABB monoclonal antibodies produced from an ovarian immunogen comprising reacting HABB monoclonal antibodies produced from an ovarian immunogen with human salivary amylase and with ovarian tumor amylase but not with human pancreatic amylase in a biological sample, and measuring the amylase remaining in the sample.
Dependencies are inferred from the claim text.