Protein purification
AssigneeGenzyme Corporation
InventorsJames R. Zabrecky, Nicholas C. Masiello
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Abstract
A method of removing an organic solvent from a mixture that includes a compound having a polypeptide segment and the organic solvent, the method including the steps of contacting the mixture with an ion exchange resin under conditions that allow the compound to bond to the resin; and washing the resin with a first aqueous solution that elutes the organic solvent from the resin while allowing the compound to remain bound to the resin.
Claims16
A method of removing an organic solvent from a mixture comprising a compound comprising a polypeptide segment and said organic solvent, said organic solvent having been introduced in a previous purifications type, said method comprising,
- contacting said mixture with an ion exchange resin under conditions that allow said compound to bond to said resin, and
- washing said resin with a first aqueous solution which contains none of said organic solvent, and that washes said organic solvent from said resin while allowing said compound to remain bound to said resin.
A method of purifying a compound which includes a polypeptide segment from a mixture, the method comprising
- subjecting said mixture to a reverse phase HPLC column and eluting a sample comprising said compound and an organic solvent from said column,
- contacting said compound and said organic solvent with an ion exchange resin under conditions that allow said compound to bond to said resin;
- and
- washing said organic solvent from said resin with an aqueous solution.
Dependencies are inferred from the claim text.