Glycoprotein and gene coding therefor
AssigneesHidechika Okada, Mitsubishi Corporation
InventorsHidechika Okada, Noriko Okada, Yoichi Nagami, Kazuhiro Takahashi, Hisao Takizawa, Jun Kondo
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Abstract
The present invention provides a glycoprotein derived from human cell membrane, which has a molecular weight of 20 to 25 Kd as estimated by SDS polyacrylamide gel electrophoresis, and contains N-glycoside type carbohydrate chain and phosphatidylinositol, and possesses an inhibitory activity to complement-mediated cell membrane damage. The present invention further provides a gene coding for the glycoprotein, and a method for the production of the glycoprotein and the gene therefor.
Claims1
A substantially pure glycoprotein free of human cell membranes, derived from human cell membranes, which has a molecular weight of 20 to 25 Kd as estimated by SDS polyacrylamide gel electrophoresis under either non-reducing or reducing conditions, and contains carbohydrate chains with at least one N-glycoside and phosphatidylinositol, and which inhibits complement-mediated cell membrane damage, and wherein said glycoprotein has the following amino acid sequence:
- ______________________________________ Met Gly Ile Gln Gly Gly Ser Val Leu Phe Gly Leu Leu Leu Val Leu Ala Val Phe Cys His Ser Gly His Ser Leu Gln Cys Tyr Asn Cys Pro Asn Pro Thr Ala Asp Cys Lys Thr Ala Val Asn Cys Ser Ser Asp Phe Asp Ala Cys Leu Ile Thr Lys Ala Gly Leu Gln Val Tyr Asn Lys Cys Trp Lys Phe Glu His Cys Asn Phe Asn Asp Val Thr Thr Arg Leu Arg Glu Asn Glu Leu Thr Tyr Tyr Cys Cys Lys Lys Asp Leu Cys Asn Phe Asn Glu Gln Leu Glu Asn Gly Gly Thr Ser Leu Ser Glu Lys Thr Val Leu Leu Leu Val Thr Pro Phe Leu Ala Ala Ala Trp Ser Leu His Pro. ______________________________________
Dependencies are inferred from the claim text.