Human relaxin polypeptides
AssigneeHoward Florey Institute of Experimental Physiology and Medicine
InventorsPeter J. Hudson, Hugh D. Niall, Geoffrey W. Tregear
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Abstract
Genes and DNA transfer vectors for the expression of human preprorelaxin; sub-units thereof, including genes and transfer vectors for expression of human prorelaxin and the individual A, B and C peptide chains thereof; and equivalents of all such genes. Methods for synthesis of the peptides involving recombinant DNA techniques.
Claims10
Essentially pure human H2-preprorelaxin, which is free of other human proteins.
Essentially pure human H2-prorelaxin, which is free of other human proteins.
An essentially pure polypeptide, wherein said polypeptide comprises:
- (i) a human H2-relaxin A chain selected from the group consisting of A(1-24) to A(5-24), wherein amino acids 1-24 have the following sequence:
- ##STR3## (ii) a human H2-relaxin B chain selected from the group consisting of b(-1-32) to B(4-23), wherein amino acids -1-32 have the following sequence:
- ##STR4## (iii) a human H2-prorelaxin C chain having the amino acid sequence as set forth in FIGS. 2A through 2D as arranged in FIG. 2, wherein the C chain amino acid sequence is modified at the junction of the B/C and C/A chains to facilitate cleavage at the B/C and C/A junctions and subsequent excision of the C chain.
An essentially pure polypeptide selected from the group consisting of signal, A, B, and C polypeptide chains of human H2-preprorelaxins, which is free of other human proteins.
Dependencies are inferred from the claim text.