Process for the production and extraction of antioxidants from a micro-organism culture
AssigneeCommissariat a l'Energie Atomique
InventorsClaude Gudin, Catherine Thepenier
Life of this patent
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Abstract
The process consists of culturing in a closed photobioreactor (2) microalgae suspended in a culture medium (1), the oxygen produced by the microalgae by photosynthesis being collected and then reinjected into the culture medium, separating (6) the microalgae from the culture medium (1), dissolving (12) the microalgae, grinding or crushing (14) the solution of microalgae, adding (16) a solvent to the ground or crushed solution for solubilizing the antioxidants produced by the microalgae and then separating (18) the liquid phases present.
Claims17
Process for the production and extraction of antioxidants, comprising the following stages:
- (a) culturing in a closed photobioreactor of photosynthetic microorganisms suspended in a liquid culture medium for producing oxygen and antioxidants, the oxygen produced by the microorganisms by photosynthesis being collected and reinjected into the culture medium, said microorganisms being chosen from the group consisting of rhodophyceae and chlorophyceae, said antioxidants being chosen from the group consisting of superoxide dismutase, vitamin C and tocopherols,
- (b) separating the microorganisms from the culture medium,
- (c) dispersing in a solution the microorganisms separated in (b),
- (d) crushing the dispersed microorganisms,
- (e) adding solvent to the solution obtained in (d) in order to solubilize the antioxidants produced by the microorganisms and then
- (f) separating the liquid and solid phases present.
A process for the production and extraction of antioxidants, comprising the following steps:
- (a) culturing in a closed photobioreactor of photosynthetic microorganisms suspended in a liquid culture medium for producing oxygen and antioxidants, collecting the oxygen produced by the microorganisms by photosynthesis and reinjecting the collected oxygen into the culture medium, heating said culture medium to increase production of antioxidants, said microorganisms being chosen form the group consisting of rhodophyceae and chlorophyceae, said antioxidants being chosen from the group consisting of superoxide dismutase, vitamin C and tocopherols,
- (b) separating the microorganisms from the culture medium,
- (c) dispersing in a solution the microorganisms separated in step (b),
- (d) crushing the dispersed microorganisms,
- (e) adding solvent to the solution obtained in (d) in order to solubilize the antioxidants produced by the microorganisms and then
- (f) separating the liquid and solid phases present.
A process for the production and extraction or antioxidants, comprising the following steps:
- (a) culturing in a closed photobioreactor of photosynthetic microorganisms suspended in a liquid culture medium for producing oxygen and antioxidants, collecting the oxygen produced by the microorganisms by photosynthesis and reinjecting the collected oxygen into the culture medium, subjecting said culture medium ionization to increase production of antioxidants, said microorganisms being chosen from the group consisting of rhodophyceae and chlorophyceae, said antioxidants being chosen from the group consisting of superoxide dismutase, vitamin C and tocopherols,
- (b) separating the microorganisms from the culture medium,
- (c) dispersing in a solution the microorganisms separated in step (b),
- (d) crushing the dispersed microorganisms,
- (e) adding solvent to the solution obtained in (d) in order to solubilize the antioxidants produced by the microorganisms, and then
- (f) separating the liquid and solid phases present.
Dependencies are inferred from the claim text.