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US 5,179,011 AGranted
Process for producing biotin vitamers using novel microorganisms
AssigneeShiseido Company, Ltd.
InventorsJiro Kishimoto, Shinichiro Haze, Ohji Ifuku
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Abstract
A mutant strain having a glucose consumption rate is at most 1/4 that of wild type strains is provided. The mutant strain belongs to Escherichia, Bacillus, Pseudomonas, or Serratia; has a glucose consumption rate of at most one fourth that of the corresponding wild type strain, and the feedback repression by biotin is removed. Further, a process for producing biotins using this mutant strain is provided.
Claims7
A process for producing biotin vitamers which comprises
- (a) cultivating in a nutrient medium a mutant strain of E. coli having
- (i) a glucose consumption rate of at most 1/4 the rate of the corresponding wild strain,
- (ii) feedback repression by biotin removed, and operon originating from E. coli inserted in the vector DNA;
- and
- (b) collecting biotin and/or desthiobiotin formed and accumulated in the culture broth;
- wherein said process is characterized by the total production of desthiobiotin and biotin in an amount at least about 3.5 times greater than the corresponding parent strain of E. coli.
3 more elements
Dependencies are inferred from the claim text.