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US 5,179,010 AGranted
Fermentation process for producing L-lysine
AssigneeAjinomoto Co., Inc.
InventorsYasuhiko Yoshihara, Yoshio Kawahara, Shigeho Ikeda
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Abstract
A fermentative process for producing L-lysine is disclosed. The process is based on growing in a culture medium a mutant strain of the genus Brevibacterium or Corynebacterium (1) capable of producing L-lysine, and (2) having an intensified superoxide dismutase activity.
Claims4
A process for producing L-lysine, which comprises:
- i) growing in an aqueous nutrient medium containing assimilable sources of carbon, nitrogen and inorganic substances the mutant strain Brevibacterium lactofermentum AJ 12220 (FERM BP-996) which is (1) capable of producing L-lysine, and (2) resistant to methyl viologen at a concentration of 1.5 .mu.g/ml;
- and
- ii) collecting L-lysine formed.
A process for producing L-lysine, which comprises:
- i) growing in an aqueous nutrient medium containing assimilable sources of carbon, nitrogen and inorganic substances the mutant strain Corynebacterium acetoglutamicum AJ 12223 (FERM BP-998) which is (1) capable of producing L-lysine, and (2) resistant to puromycin at a concentration of 0.5 .mu.g/ml;
- and
- ii) collecting L-lysine formed.
A process for producing L-lysine, which comprises:
- i) growing in an aqueous nutrient medium containing assimilable sources of carbon, nitrogen and inorganic substances the mutant strain Brevibacterium flavum AJ 12222 (FERM BP-997) which is (1) capable of producing L-lysine, and (2) resistant to phenylhydrazine at a concentration of 20 .mu.g/ml;
- and
- ii) collecting L-lysine formed.
A process for producing L-lysine, which comprises:
- i) growing in an aqueous nutrient medium containing assimilable sources of carbon, nitrogen and inorganic substances the mutant strain Brevibacterium lactofermentum AJ 12221 (FERM BP-2329) which is (1) capable of producing L-lysine, and (2) resistant to benzoyl peroxide at a concentration of 20 .mu.g/ml;
- and
- ii) collecting L-lysine formed.
Dependencies are inferred from the claim text.