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US 5,179,009 AGranted
Process for producing L-aspartyl-L-phenylalanine and its diketopiperazine
AssigneeAjinomoto Co., Inc.
InventorsKenzo Yokozeki, Naoki Usui, Toshihide Yukawa, Yoshiteru Hirose, Koji Kubota
Life of this patent
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Abstract
The present invention is directed to a process for producing L-aspartyl-L-phenylalanine and/or L-aspartyl-L-phenylalanine diketopiperazine comprising contacting and reacting a culture, cell or a cell-treated product of a microorganism capable of hydrolyzing DKP into AP or capable of performing intramolecular condensation of AP into DKP with DKP and/or AP, in an aqueous medium.
Claims13
A process for producing L-aspartyl-L-phenylalanine, which comprises:
- contacting a microorganism selected from the group consisting of Streptomyces flavovirens IFO accession no. 3197, Achromobacter lacticum FERM-P accession no. 7401, Agrobacterium tumefaciens ATCC accession no. 4452, Alcaligenes faecalis FERM-P accession no. 8460, Bacillus circulans ATCC accession no. 9966, Enterobacter agglomerans ATCC accession no. 12287, Ervinia amylovora FERM-P accession no. 7056, Flavobacterium rhenanum FERM-P accession no. 8459, Micrococcus varians ATCC accession no. 399, Serratia marcescens FERM-P accession no. 8461 and Santhomonas citri FERM-P accession no. 8462, with L-aspartyl-L-phenylalanine diketopiperazine in an aqueous medium under aerobic conditions, a pH of about 2-8 and a temperature of about 20.degree.-40.degree. C. for a time sufficient to produce L-aspartyl-L-phenylalanine, wherein said microorganism enzymatically hydrolyzes L-aspartyl-L-phenylalanine diketopiperazine into L-aspartyl-L-phenylalanine and recovering the L-aspartyl-L-phenylalanine product.
A process for producing L-aspartyl-L-phenylalanine diketopiperazine, which comprises:
- contacting a microorganism selected from the group consisting of Streptomyces flavovirens IFO accession no. 3197, Achromobacter lacticum FERM-P accession no. 7401, Agrobacterium tumefaciens ATCC accession no. 4452, Alcaligenes faecalis FERM-P accession no. 8460, Bacillus circulans ATCC accession no. 9966, Enterobacter agglomerans ATCC accession no. 12287, Ervinia amylovora FERM-P accession no. 7056, Flavobacterium rhenanum FERM-P accession no. 8459, Micrococcus varians ATCC accession no. 399, Serratia marcescens FERM-P accession no. 8461 and Xanthomonas citri FERM-P accession no. 8462, with L-aspartyl-L-phenylalanine under aerobic conditions, a pH of about 6 and a temperature of about 20.degree.-40.degree. C. for a time sufficient to produce L-aspartyl-L-phenylalanine diketopiperazine, wherein said microorganism performs the enzymatic intramolecular condensation of L-aspartyl-L-phenylalanine to produce L-aspartyl-L-phenylalanine diketopiperazine, and recovering the L-aspartyl-L-phenylalanine diketopiperazine product.
Dependencies are inferred from the claim text.