Process for the production of proteins or protein-containing gene products
AssigneeBoehringer Mannheim GmbH
InventorsDieter H. Wolf, Erhard Kopetzki, Gunther Schumacher
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Abstract
The present invention provides a process for the production of proteins or protein-containing gene products by transformation of eukaryotic host cells with a recombinant DNA molecule containing the gene for the desired protein, culturing the cells and isolating the gene product after expression, wherein, as host cells, there is used a yeast strain which is deficient in proteases A and B.
Claims11
In a process for production of a protein or protein-containing cell product wherein
- yeast cells are transformed with a recombinant DNA molecule containing a gene which codes for said protein or protein contained in said protein-containing cell product, fermenting said cells under conditions favoring expression of said protein or protein in said protein-containing cell product, and
- isolating said protein or protein-containing cell product the improvement consisting of transforming yeast cells of a YEp- or YRp- strain deficient in vacuolar proteases A, B, and one or more proteases selected from the group consisting of carboxypeptidases Y and S to prevent product degradation.
Process for the production of a protein or protein containing cell product comprising crossing a yeast strain deficient in vacuolar proteases A and B with another yeast strain which is auxotrophic or chemically-sensitive, sporulating the product of said crossing, selecting a hybrid yeast strain which is deficient in proteases A and B and is further characterized by at least one auxotrophy or chemical sensitivity of one of the parent strains, transforming said hybrid strain with a recombinant DNA molecule containing a gene which codes for said protein or protein in said protein containing cell product, fermenting said hybrid strain under conditions favoring expression of said protein or said protein in said protein containing cell product and isolating said protein or protein containing cell product.
Dependencies are inferred from the claim text.