Selective immobilization and detection of mycotoxins in solution

Apparatus for the separation and detection of aflatoxin and ochratoxin A from a solution which comprises:

• a transparent tube containing a sequence of discrete layers including:
• (1) A first adsorbent layer having a structure-selective affinity for ochratoxin A, but not aflatoxin;
• (2) A second adsorbent layer having a structure-selective affinity for aflatoxin;
• and
• (3) A support layer between the first and second adsorbent layers;
• said support layer being made of material other than those of said first and second adsorbent layers.

Apparatus for the separation and detection of ochratoxin A, zearalenone, and aflatoxin from a solution which comprises:

• a transparent tube containing a sequence of discrete layers including:
• (1) A first adsorbent layer having a structure-selective affinity for ochralayer A, but not zearalenone or aflatoxin;
• (2) A second adsorbent layer having a structure-selective affinity for zearalenone, but not aflatoxin;
• (3) A third adsorbent layer having a structure-selective affinity for aflatoxin;
• (4) A first support layer between the first and second adsorbent layers;
• said first support layer being made of material other than those of said first, second and third adsorbent layers;
• and
• (5) A second support layer between the second and third adsorbent layers;
• said second support layer being made of material other than those of said first, second and third adsorbent layers.

An apparatus for the separation and detection of zearalenone and aflatoxin from a solution which comprises:

• a transparent tube containing a sequence of discrete layers including:
• (1) A first adsorbent layer having a structure-selective affinity for zearalenone, but not aflatoxin, said first adsorbent being capable of immobilizing said zearalenone in the presence of an appropriate solvent, but not aflatoxin;
• (2) A second adsorbent layer having a structure-selective affinity for aflatoxin, said second adsorbent layer being capable of immobilizing said aflatoxin in the presence of an appropriate solvent;
• (3) A first support layer between the first and second adsorbent layers;
• said first support layer being made of material other than those of said first and second adsorbent layers.

Apparatus for the separation and detection of sterigmatocystin and aflatoxin-M1 which comprises:

• a transparent tube containing a sequence of discrete layers including:
• (1) A first adsorbent layer having a structure-selective affinity for sterigmatocystin, but not aflatoxin-M1;
• (2) A second adsorbent layer having a structure-selective affinity for aflatoxin-M1;
• and
• (3) A first support layer between the first and second adsorbent layers;
• said first support layer being made of material other than those of said first and second adsorbent layers.

Apparatus for the separation and detection of aflatoxin and deoxynivalenol from a solution which comprises:

• a transparent tube containing a sequence of discrete layers including:
• (1) A first adsorbent layer having a structure-selective affinity for aflatoxin, but not deoxynivalenol;
• (2) A second adsorbent layer having a structure-selective affinity for deoxynivalenol;
• and
• (3) A first support layer between the first and second adsorbent layers;
• said first support layer being made of material other than those of said first and second adsorbent layers.

Apparatus for the separation and detection of sterigmatocystin from a solution which may contain interfering materials comprising:

• a transparent tube containing a sequence of discrete layers including:
• (1) A first adsorbent layer which is capable of selectively binding interfering materials from the solution as it passes through the tube;
• (2) A second adsorbent layer having a structure-selective affinity for sterigmatocystin;
• and
• (3) A support layer between said first and second adsorbent layers;
• said support layer being made of material other than those of said first and second adsorbent layers.

Apparatus for separation and detection of patulin from a solution which may contain interfering materials comprising:

• a transparent tube containing a sequence of discrete layers including:
• (1) A first adsorbent layer which is capable of selectively binding interfering materials from the solution as it passes through the tube;
• (2) A second adsorbent layer having a structure-selective affinity for patulin;
• and
• (3) A support layer between the first and second adsorbent layers;
• said support layer being made of material other than those of said first and second adsorbent layers.

Apparatus for separation and detection of citrinin from a solution which may contain interfering materials comprising:

• a transparent tube containing a sequence of discrete layers including:
• (1) A first adsorbent layer which is capable of selectively binding interfering materials from the solution as it passes through the tube;
• (2) A second adsorbent layer having a structure-selective affinity for citrinin;
• and
• (3) A support layer between the first and second adsorbent layers;
• said support layer being made of material other than those of said first and second adsorbent layers.

Apparatus for separation and detection of ergots from a solution which may contain interfering materials comprising:

• a transparent tube containing a sequence of discrete layers including:
• (1) A first adsorbent layer which is capable of selectively binding interfering materials from the solution as it passes through the tube;
• (2) A second adsorbent layer having a structure-selective affinity for ergots;
• and
• (3) A support layer between the first and second adsorbent layers;
• said support layer being made of material other than those of said first and second adsorbent layers.

A kit for selective separation and detection of a mycotoxin from a solution which may contain interfering materials comprising:

• a transparent tube containing a sequence of discrete layers including:
• (1) A first adsorbent layer which is capable of selectively binding interfering materials from the solution as it passes through the tube;
• (2) A second adsorbent layer having a structure-selective affinity for a mycotoxin said second adsorbent layer being capable of immobilizing said mycotoxin in the presence of an appropriate solvent;
• (3) A support layer between the first and second adsorbent layers;
• said support layer being made of material other than those of said first and second adsorbent layers;
• and
• an appropriate solvent.

A kit for the separation and detection of zearalenone and aflatoxin from a solution comprising:

• a transparent tube containing a sequence of discrete layers including:
• (1) A first adsorbent layer having a structure-selectively affinity for zearalenone, but not aflatoxin, said first adsorbent being capable of immobilizing said zearalenone in the presence of an appropriate solvent, but not aflatoxin;
• (2) A second adsorbent layer having a structure-selective affinity for aflatoxin, said second adsorbent layer being capable of immobilizing said aflatoxin in the presence of an appropriate solvent;
• (3) A first support layer between the first and second adsorbent layers;
• said first support layer being made of material other than those of said first and second adsorbent layers;
• and
• an appropriate solvent.