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US 5,177,307 AGranted
Compositions and methods for modulation of endogenous cytokinin levels
AssigneeCalgene, Inc.
InventorsCatherine M. Houck, Julie R. Pear, Belinda M. Martineau, William Hiatt
Life of this patent
Estimating the term…
Abstract
Developmentally regulated transcriptional regulatory regions are identified employing cDNA screening. The resulting regulatory regions are manipulated for use with DNA sequences encoding enzymes involved in cytokinin metabolism for introduction into plant cells to provide transformed plants having tissue, particularly fruit, with a modified phenotypic property.
Claims17
An expression cassette comprising:
- in the 5'-3' direction of transcription, as operably joined components, a transcriptional and translational initiation region from a gene selected from the group consisting of 2All, Z130 and Z70, a DNA sequence encoding an enzyme in a cytokinin metabolic pathway, wherein transcription of said DNA sequence is regulated by said transcriptional initiation region, and a transcriptional termination region, wherein at least one of said initiation and termination regions is heterologous to said DNA sequence.
A tomato plant cell comprising:
- an expression cassette comprising, in the 5'-3' direction of transcription as operably joined components, a transcriptional and translational initiation region from a gene selected from the group consisting of 2All, Z130 and Z70, a DNA sequence encoding an enzyme in a cytokinin metabolic pathway, wherein said DNA sequence is under the transcriptional regulation of said transcriptional initiation region, and a transcriptional termination region, wherein at least one of said initiation and termination regions is heterologous to said DNA sequence.
A method of modifying the phenotype of a tomato plant, said method comprising:
- growing a tomato plant comprising cells transformed with an expression cassette comprising, in the 5'-3' direction of transcription as operably joined components, a transcriptional and translational initiation region from a gene selected from the group consisting of 2All, Z130 and Z70, a DNA sequence encoding an enzyme in a cytokinin metabolic pathway, wherein said DNA sequence is under the transcriptional regulation of said transcriptional initiation region, and a transcriptional termination region, wherein at least one of said initiation and termination regions is heterologous to said DNA sequence.
A tomato fruit having a modified phenotype said fruit comprising:
- plant cells containing an expression cassette comprising in the 5'.fwdarw.3' direction of transcription as operably joined components, a transcriptional and translational initiation region from a gene selected from the group consisting of 2All, Z130 and Z70, a DNA sequence encoding an enzyme in a cytokinin metabolic pathway, wherein transcription of said DNA sequence is regulated by said transcriptional initiation region, and a transcriptional termination region, wherein at least one of said initiation and termination regions is heterologous to said DNA sequence.
Dependencies are inferred from the claim text.