Determining cereal quality
AssigneeAgricultural & Food Research Counsel
InventorsHenry W. Chan, Michael R. A. Morgan, Elizabeth N. C. Mills
Life of this patent
Estimating the term…
Abstract
The three amino acid sequence TCP (threonine-crysteineproline) has been found, in wheat glutenin, to correspond to good bread-making qualities in wheat flour. This sequence, and 32 nine amino acid sequences containing it, can be detected by amino acid analysis of the glutenin, by specific monoclonal antibodies or by analysis (e.g. nucleotide sequence analysis or hybridization assays) of the wheat genome.
Claims4
A method of determining wheat characteristics comprising the steps of:
- contacting wheat glutenin with an antibody that specifically binds an amino acid sequence selected from the group consisting of GSVTCPQQV, GSVSCPQQV, GTVTCPQQV, GSVTCPQQA, GSTTCPQQV, GSVTCPQQT, GLSTCPQQV, GSVTCPQQL, GAVTCPQQV, GSVACPQQV, GSVGCPQQV, GSVNCPQQV, GSVTCPQPV, GSVTCPQQI, GSVTCPQQL, VQQPCTVSG, substitutions of the foregoing sequences wherein a terminal glycine is substituted with proline, TCP, and PSVTCPQQV;
- and detecting the formation of an immune complex between said antibody and the wheat glutenin.
A composition consisting essentially of an isolated and purified antibody that specifically binds to a sequence selected from the group consisting of:
- GSVTCPQQV, GSVSCPQQV, GTVTCPQQV, GSVTCPQQA, GSTTCPQQV, GSVTCPQQT, GLSTCPQQV, GSVTCPQQL, GAVTCPQQV, GASVACPQQV, GSVGCPQQV, GSVNCPQQV, GSVTCPQPV, GSVTCPQQI, GSVTCPQQL, VQQPCTVSG;
- substitutions of the foregoing sequences wherein a terminal glycine is substituted with proline;
- TCP;
- and PSVTCPQQV.
Dependencies are inferred from the claim text.