Preparation of an immobilized lipase having a low water content without drying
AssigneeAjinomoto Co., Inc.
InventorsNaoki Usui, Naoto Kato, Tsuyoshi Nakamatsu, Jun Kurashige
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Abstract
Lipase is absorbed on a carrier to provide an immobilized lipase preparation containing 0.5 to 8% water by a process that does not require drying. The process is carried out by providing a solution or dispersion of lipase in a liquid solvent, mixing the solution or dispersion of lipase with a carrier to provide a mixture containing 0.5 to 8% water and kneading the mixture to produce the immobilized lipase preparation. The solution or dispersion of lipase may contain an enzyme activity enhancing agent such as a fatty acid or its derivative such as lecithin. By not drying, an immobilized lipase preparation having higher activity is obtained.
Claims21
A process of preparing an immobilized lipase preparation having a final water content of 0.5 to 8% without drying wherein the lipase is absorbed on a carrier, consisting essentially of the steps of:
- (A) providing a solution or dispersion of lipase in a liquid solvent;
- and
- (B) mixing the solution or dispersion of lipase with a carrier to provide a mixture containing 0.5 to 8% water and kneading the mixture to produce said immobilized lipase preparation.
Dependencies are inferred from the claim text.