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US 5,177,011 AGranted
Plant elongation factor promoters, coding sequences and uses
AssigneeCalgene, Inc.
InventorsChristine K. Shewmaker, William R. Hiatt, Ann R. Pokalsky
Life of this patent
Estimating the term…
Abstract
Expression constructs are provided employing a plant EF-1.alpha. promote which allows for elevated expression in rapidly dividing cells. Sequences from the gene and untranslated regions associated with the gene may be employed in an antisense construct to reduce growth rate. The promoter finds particular use in protecting rapidly dividing tissue and tender shoots from a wide variety of environmentally induced stress conditions.
Claims2
A method to preferentially expose rapidly dividing plant cells to an expression product as distinct from other plant cells, said method comprising,
- growing a plant comprising cells containing a DNA construct integrated in the genome of said cells, said DNA construct comprising as operably joined components, in the 5' to 3' direction of transcription, a tomato elongation factor-1.alpha. transcriptional initiation region, a tomato elongation factor-1.alpha. translational initiation region, a foreign DNA sequence, and a transcriptional termination region functional in a plant.
Dependencies are inferred from the claim text.