In vitro enzymatic conversion of glycosylated mammalian vitamin D-binding protein to a potent macrophage activating factor
AssigneeNobuto Yamamoto
InventorNobuto Yamamoto
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Abstract
A novel macrophage activating factor is prepared in vitro by treating glycosylated mammalian vitamin D-binding protein with glycosidases. Vitamin D-binding protein, which is isolated from blood or plasma of animals by known procedures, is thus readily converted to a highly potent macrophage activating factor.
Claims34
A process for producing a macrophage activating factor comprising contacting glycosylated mammalian vitamin D-binding protein in vitro with
- .beta.-galactosidase, or
- .beta.-galactosidase in combination with sialidase, .alpha.-mannosidase, or a mixture thereof,
- and isolating the macrophage activating factor.
A macrophage activating composition comprising, in combination with a pharmaceutically acceptable carrier, a macrophage activating factor formed by treating glycosylated vitamin D-binding protein in vitro with
- .beta.-galactosidase, or
- .beta.-galactosidase in combination with sialidase, .alpha.-mannosidase, or mixtures thereof.
A method for inducing macrophage activation in an mammal in need thereof comprising administering to such mammal a macrophage activating factor prepared by contacting glycosylated mammalian vitamin D-binding protein in vitro with
- .beta.-galactosidase, or
- .beta.-galactosidase in combination with sialidase, .alpha.-mannosidase, or a mixture thereof.
Dependencies are inferred from the claim text.