Back to results
US 5,176,997 AGranted
DNA probe for male infertility
AssigneeUniversity of Florida
InventorMichael G. Humphreys-Beher
Life of this patent
Estimating the term…
Abstract
The subject invention pertains to a novel means of identifying male infertility. The novel method involves the identification of a unique restriction enzyme digestion pattern which is highly specific to individuals with male sperm binding infertility.
Claims8
A method for detecting human male fertility or infertility, said method comprising:
- (a) isolating DNA from said human male;
- (b) digesting the isolated DNA with the restriction endonuclease, TaqI;
- (c) electrophoresing the digested DNA on an agarose gel;
- (d) hybridizing the electrophoresed DNA with a probe comprising a cDNA clone having the nucleotide sequence of the full length cDNA insert coding for human galactosyltransferase activator from ATCC 68007;
- and
- (e) identifying a unique restriction fragment pattern, wherein said fragments are characteristic of said TaqI restriction endonuclease and said pattern is characteristic of either fertility or infertility.
3 more elements
A kit for detecting human male infertility comprising cDNA which hybridizes with the gene coding for galactosyltransferase activator wherein said cDNA has the sequence shown in SEQ ID NO. 1 or a 1.0 kilobase 3' fragment of the sequence shown in SEQ ID NO. 1.
Dependencies are inferred from the claim text.