Transgenic mice depleted in mature T-cells and methods for making transgenic mice
AssigneeGenPharm International, Inc.
InventorsPaulus J. A. Krimpenfort, Antonius J. M. Berns
Life of this patent
Estimating the term…
Abstract
Transgenic mice having a phenotype characterized by the substantial absence of mature T lymphocytes otherwise naturally occurring in said mice. The phenotype is conferred in the transgenic mouse by a transgene contained in at least the precursor stem cell of the T lymphocyte which is depleted. The transgene comprises a DNA sequence encoding a T cell receptor polypeptide variant which inhibits maturation of the T lymphocyte.
Claims5
A transgenic mouse having a phenotype characterized by the substantial absence of mature T-cells otherwise naturally occurring in said mouse, said phenotype being conferred by a transgene contained in the somatic and germ cells of said mouse, said transgene comprising the .DELTA.V-TCR.beta. DNA fragment which encodes a T-cell antigen receptor polypeptide variant, and said polypeptide variant being incapable of mediating T-cell maturation in said transgenic mouse.
A method for producing a transgenic mouse having a phenotype characterized by the substantial absence of mature T-cells otherwise naturally occurring in said mouse, said method comprising:
- (a) introducing a transgene into a zygote of a mouse, said transgene comprising the .DELTA.V-TCR.beta. DNA fragment which encodes a T-cell antigen receptor polypeptide variant, said polypeptide variant being incapable of mediating T-cell maturation in said transgenic mouse,
- (b) transplanting said zygote into a pseudopregnant mouse.
- (c) allowing said zygote to develop to term, and
- (d) identifying at least one transgenic offspring containing said transgene.
A method for producing a transgenic mouse having a phenotype characterized by the substantial absence of mature T-cells otherwise naturally occurring in said mouse, said method comprising:
- (a) introducing a transgene into an embryo of a mouse, said transgene comprising the .DELTA.V-TCR.beta. DNA fragment which encodes a T-cell antigen receptor polypeptide variant, said polypeptide variant being incapable of mediating T-cell maturation in said transgenic mouse.
- (b) transplanting said embryo into a pseudopregnant mouse,
- (c) allowing said embryo to develop to term, and
- (d) identifying at least one transgenic offspring containing said transgene, and
- (e) breeding said offspring to form a transgenic mouse having said phenotype.
Dependencies are inferred from the claim text.