Methods for purification of platelet-derived growth factor
AssigneeAmgen Inc.
InventorsArlen R. Thomason, Margery A. Nicolson
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Abstract
Monoclonal antibodies specific for epitopes found on the B chain of PDGF (including v-sis, c-sis and platelet-derived forms) may be bound to columns and used for purification of rPDGF B. A solution containing a polypeptide possessing at least part of the structural conformation of rPDGF B is passed over such a column and the rPDGF B is bound to the antibody. The rPDGF B may then be eluted from the column to yield rPDGF B of greater than 95% purity as determined by SDS-PAGE.
Claims18
A method for purifying a polypeptide having a sufficient part of the structural conformation of rPDGF B to provide at least one epitope found in rPDGF B.sub.c-sis or PDGF B.sub.v-sis comprising the steps of:
- (a) contacting a substrate-bound monoclonal antibody specific for an epitope found in the B chain of PDGF with a solution containing said polypeptide and
- (b) eluting said polypeptide from said substrate-bound monoclonal antibody.
A method for purifying a polypeptide having a sufficient part of the structural conformation of rPDGF B to provide at least one epitope found in rPDGF B.sub.c-sis or PDGF B.sub.v-sis comprising the steps of:
- (a) contacting a substrate-bound monoclonal antibody specific for an epitope found in the B chain of PDGF but not the A chain of PDGF with a solution containing said polypeptide and
- (b) eluting said polypeptide from said substrate-bound monoclonal antibody.
Dependencies are inferred from the claim text.