Plasmids from corynebacterium glutamicum and plasmid vectors derived therefrom
AssigneeDegussa AG
InventorsFrank Bachmann, Hans J. Kutzner, Hans Sonnen, Georg Thierbach, Petra-Sabine Kautz, Alfred Puhler, Andreas Schaefer
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Abstract
The present invention relates to new plasmids isolated from Corynebacterium glutamicum, plasmid pGA1 and pGA2, which are compatible with one another. In addition, the present invention relates to recombinant plasmids and to plasmid vectors (shuttle vectors) containing the new plasmids. The plasmids and plasmid vectors of the present invention are suitable for use with genetic engineering to improve bacteria strains.
Claims9
A plasmid pGA1, isolated from Corynebacterium glutamicum LP-6 (deposited under number DSM 5816) characterized by a length of .about.4.9 kb and the following restriction cleavage sites:
- ______________________________________ Restriction Number of DNA fragments enzymes cleavage sites (kb) ______________________________________ Apa I 0 -- Bam H I 2 3,3 1,6 Bcl I 0 -- Bgl I 0 -- Bgl II 0 -- Bst E II 1 4,9 Cla I 1 4,9 Dra I 0 -- Eco R I 1 4,9 Eco R V 0 -- Hind III 4 2,4 1,1 1,05 0,3 Kpn I 0 -- Mlu I 3 2,45 2,25 0,2 Pvu II 2 3,25 1,65 Sal I 0 -- Sph I 1 4,9 Sst I 0 -- Xba I 2 2,5 2,4 Xho I 0 -- ______________________________________
A plasmid pGA2, isolated from Corynebacterium glutamicum LP-6 (deposited under number DSM 5816) characterized by a length of .about.19.5 kb and the following restriction cleavage sites:
- ______________________________________ Restriction Number of DNA fragment enzymes cleavage sites (kb) ______________________________________ Bam H I 3 13,6 4,15 1,75 Eco R I 2 18,5 0,96 Hind III 5 6,61 5,72 3,31 2,04 1,82 Hpa I 1 19,5 Kpn I 1 19,5 Mlu I 3 15,8 2,84 0,82 Sca I 1 19,5 Sma I 4 14,5 3,34 1,00 0,65 Xho I 0 -- ______________________________________
Dependencies are inferred from the claim text.