Process for the production of urokinase using Saccharomyes cerevisiae
AssigneeCiba-Geigy Corporation
InventorsBernd Meyhack, Jutta Heim, Rolf Burgi
Life of this patent
Estimating the term…
Abstract
Novel human plasminogen activators of the urokinase type are produced by yeast cells transformed with a hybrid vector comprising a DNA sequence coding for said human plasminogen activator. Novel hybrid vectors, yeast hosts transformed with such hybrid vectors and processes for the production thereof are also provided. 77
Claims4
A method for the production of biologically active human single chain urokinase-type plasminogen activator the S. cerevisiae GAPDH-promoter, a DNA segment consisting of a firs DNA sequence encoding the invertase signal sequence, upstream of and in reading frame with a second DNA sequence coding for said urokinase-type plasminogen activator, which DNA segment is under transcriptional control of said GAPDH-promoter, and a DNA sequence comprising transcription termination signals of the S. cerevisiae PH05 gene, and isolating said urokinase-type plasminogen activator from the cell interior.
A hybrid vector for use in S. cerevisiae, comprising an expression cassette consisting of the S. cerevisiae GAPDH-promoter, a DNA segment consisting of a first DNA sequence encoding the invertase signal sequence, upstream of and in reading frame with a second DNA sequence coding for human urokinase-type plasminogen activator, which DNA segment is under transcriptional control of said GAPDH-promoter, and a DNA sequence comprising transcription termination signals of the S. cerevisiae PH05 gene.
Dependencies are inferred from the claim text.