US 5,175,101 A · Recombinant restriction enzyme Sau3AI

Abstract

The invention concerns a DNA which contains (1) at least one of the two regions of the nucleotide sequence SEQ ID NO:1 coding for a protein with a Sau3AI methylase activity or for a protein with a Sau3AI endonuclease activity. (2) a sequence corresponding to the DNA from (1) within the scope of the degeneration of the genetic code or (3) a sequence which hybridizes under stringent hybridization conditions with a DNA from (1) or (2). In addition a vector is disclosed which contains a DNA according to the present invention, in particular under the control of a regulatable closed promoter, as well as microorganisms which are transformed with one or several vectors according to the present invention and the isolation of recombinant Sau3AI endonuclease from the transformed microorganisms.

Claims27

Dependent claims

DNA, comprising:

(1) at least one of the two regions of the nucleotide sequence SEQ ID NO:1 or a mutation of said nucleotide sequence coding for a protein with a Sau3AI methylase activity or for a protein with a Sau3AI endonuclease activity, or
(2) a sequence complementary to the DNA sequence from (1).

Dependencies are inferred from the claim text.

Facts

Application

07/712,833Filed 10 Jun 1991

Priority

DE 40184418 Jun 1990

Granted

29 Dec 1992

Term ends

Not estimatedThe source records don’t support an estimate.

Classification5 codes

C12N 9/1007
Transferring one-carbon groups: methyltransferases
C12N 9/22
Acting on ester bonds: ribonucleases [RNase]; Deoxyribonucleases [DNase]
C12N 15/70
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression: vectors or expression systems…
C12N 15/74
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression: vectors or expression systems…

Recombinant restriction enzyme Sau3AI

Life of this patent

Recombinant restriction enzyme Sau3AI

Life of this patent

Abstract

Claims27