Loading patent…
AssigneeGenetic Systems Corporation
InventorsSusan M. Watanabe, Wesley L. Cosand, Susan McArdle, Pamela J. Ward
Estimating the term…
Immunologically reactive gag proteins of LAV/HTLV-III are expressed in bacterial cells. The gag proteins are encoded by a recombinant plasmid containing procaryotic transcriptional and translational signals for expression, followed downstream by a DNA sequence comprising pGAG-1. Preferred signals for expression are selected from an inducible and/or suppressible operon, such as the trp operon. The gag proteins are isolated from the bacterial host and are utilized in diagnostic assays which detect the presence of LAV/HTLV-III antigens or antibodies immunologically reactive with LAV/HTLV-III. Further, the proteins produced by the method disclosed may be used as a vaccine against infection by the causative virus for acquired immune deficiency syndrome.
An isolated and purified DNA sequence comprising a portion of the gag region of the LAV/HTLV-III genome, wherein said portion corresponds to that from bp 375 to bp 961 of LAV and which codes for a protein which is immunologically reactive with antibodies to LAV/HTLV-III.
A recombinant plasmid capable of replication in bacterial host cells, said plasmid including procaryotic transcriptional and translational signals for expression, followed in reading phase by a DNA sequence comprising a portion of the gag region of the LAV/HTLV-III genome, wherein said portion corresponds to that from bp 375 to bp 961 of LAV and which codes for a protein which is immunologically reactive with antibodies to LAV/HTLV-III.
A bacterial cell transformed with a recombinant plasmid capable of replication in bacterial host cells, said plasmid including procaryotic transcriptional and translational signals for expression, followed in reading phase by a DNA sequence comprising a portion of the gag region of the LAV/HTLV-III genome, wherein said portion corresponds to that from bp 375 to bp 961 of LAV and which codes for a protein which is immunologically reactive with antibodies to LAV/HTLV-III.
A method for preparing proteins which are immunologically reactive with antibodies to LAV/HTLV-III, comprising:
Dependencies are inferred from the claim text.