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US 5,175,096 AGranted
DNA encoding a fibronectin binding protein as well as its preparation
AssigneeAlfa-Laval Agri International Aktiebolag
InventorsMagnus Hook, Klas Jonsson, Kjell M. Lindberg, Christer Signas
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Abstract
The present invention relates to a new recombinant hybrid-DNA-molecule comprising a nucleotide sequence from S. aureus coding for a protein, or polypeptide, having fibronectin binding properties.
Claims6
A hybrid DNA molecule comprising a nucleotide sequence from S. aureus coding for a protein or polypeptide having fibronectin binding activity, said nucleotide sequence comprising:
- ______________________________________ GTTAACAACA ATCTTAACTT TTTATTAACT AAAAACCGAA CAATATAGAA TTGCATTTAT ATTTTGCATT TAAGGGAGAA TATTATAGTG CATAAGAAAA CACAAATTGG GAGCGGCCTC TCGTTGTTGG AATGGGACAA GAAAAAGAAG AATACTACAG TAGAGGAAAG TGGGAGTTCA CGAAACACAA ACAACTACAA ATAACGTTAA CATACAGCGC GACATCAACT GA GCAACCAT ACAGAAGAAG CACCGAAAAC TGTGCAAGCA AGTTGATTTG CCATCGGAAA AAGTTGCTGA AAGTTGACAT AGCTCAACAA AGTAAAGTCT AAAAGATCAA CTGACGTTAC AGCAGTTGCA AACTAAAGCG ACAGGTACAG ATGTAACAAA GTAGTGAAAT TGTAGGACAT AAACAAGATA AACGCAGAAA GAGTAACCTT GAAATATAAA TAAGGCGGGA GATTATTTTG ATTTCACAT T ATGGTATCTC AACACTGCGT AAAGTTCCGG CAAGTTATGG CGACAGGAGA AATAATTGGA GTTTAAAGAA TATGTACAAG AAAAGAAAGA TAAATCTATT TATTGATCCT ACAACAGTGA GTTGAAGTTA AATTGGGTGA GACTACGGTT ATATTTAGGT GGAGTTAGAG ATAATTGGGG TTGATACTTT AAATAAAGTA GATGGGAAAT AAACCTAACA ACCAGTCGTT AAGCTCTGTG TAAAGGAAAT AA ACCAGGGG TTAATAATCC ACATTGGTTC AGACGATTTA GCTGAAAGCG GTCAGCAAAT TTGAAGATGT GACTGATAAT TATTGGTGGT TATTCTTTAA ACTTTAATAA ATGTAATAAA ATATGAAGGG TATTATGATT TTTCAAACAC ACCTTTTTGG ATATTATAAC AACTTGGAAA AATGGCGTTG CATTTTACTC GCAAAGATAA ACTAAAGGAA CCTATTATAG CTTGAATT TA AATCAGAGCC GCCAGTGGAG AATCGAAGAA AGTAATGATT CTAAGCCAAT CTGTTGAAGG TGCAGAAGGT CATGCAGAAG GA TTCTATTC ATGTAGACTT TGAAGAATCG TCATGCTGAT GTTGTTGAAT ATGAAGAAGA AGGTTACTAC TGAGTCTAAC CTAGTTGAAT GGTATTGTAA CTGGTGCTGT TAGCGATCAT AGAATATACG ACTGAAAGTA ACTTGATTGA AAGAACATGG TCAAGCGCAA GGACCA ATCG CATCATATTT CTCATTCTGG TTTAGGAACT TGGCGTGATT GAAGAAATCG AAGAAAATAG AATTAGGTTA CGAAGGTGGC CAAAATAGCG GACACAGAAG AAGATAAACC GAAATATGAA TATCGATTTC GATAGTGTAC CTCAAATTCA AATCATTCGA AGAAGATACA GAGAAAGACA GGTAATATCA TTGATATCGA CTTCGACAGT CAATAAGCAC ACTGAAATTA TTGAAGAAGA ATTATCAATT CGGTGGACAC AATAGTGTTG CCACAAGTAA GTGGTCATAA TGAAGGTCAA AACACCTCCA ATCGTGCCAC CAACGCCACC AGCCGGAAAC ACCAACACCA CCGACACCAG ACACCAACAC CGCCAACGCC AGAGGTACCA ACCACCTGCT AAAGAAGAAC CTAAAAAACC GTAAAGTAGT AACACCTGTT ATTGAAATCA GTACCAACTA AAAAAGCACA ATCTA AGAAA TGGAGAAGAA TCAACAAACA ACGGCATGTT TTTTAGGTTT AGCGTTATTA CGCAGAAATA TCAATCCAAA ATTGACAGGT TTATTTCATA TTTTTTAAAA TTAAAACAAA TTTCCCAAGA CACTATGAAG GCGTTCTAAT TAGTGTTAAA TAATACTTAG GAAAAGGAGC ACATTAACTT ATCATTCCCG CTGCTGGTTT AGGGACTAGA CGCTTTTTTT CATTGCTT TT TGAGTTTTTA AAATAAATGA AAAAGCAATC TTAGATACGG AGTATTCTTA GGAACAATGA CTGCAGCATC GGAACAAAAC GCTACTGAAA GTAAAGCAAG TACAATAGAT GAAACACAAT CACAATCAAC ACAAGTAACA CCAAAAGTAG AAACTTCGCG TAAGGAAACT ACAGGAACTC CAGAAATTAA ACCAAGAATG GAGAAAGAAG TAGTGGAAGA TAAAGTGGAA GTAGAAGAAG CGAATGTTGT AAATCCTCAT TGGAAATTTG GAGAAGGAAT AAGCGATAAT GTTGAAACTC AGATAAAAAG TACAGATGGT GAAAGAAAAG TTAGATATAC TTTAACTGCT GAATTATCTT CGCAAAAAGG TAACCAAAAT AGCAAAATAT TTAATATTCA AGTAACAGCT AATGGTCGAA TTAGTCATTT TGCGTACATG ACAGTAACTG GTCAAGTAAC AACAGTTAAG GTATATAA AC TATATGCAAA GCTTGATGAT ATGAGTTTAG ATTTTGATAC TTTAGACCAA AGTAAAAATT CAAATGCTAG CAACTTAGAA TATTATTATA CAAGTAATTT TAATAACGCT CAAGGCGACG AACATAGTAC TCCTATCGAA AAGCATGAAT TGACTGGTAC TGATTTTGAA TATCATACAG GTACCATTGA AACTGAAGAA ACACATGAAA ATTCAAAACA TACAAAC CCA GGTGGTGGTC TTGACGAAGA TTCTACAAAA ACAACAATTG AAGATACGAA ACTAGTAGAT GAACTACCTG AGGAAATTAC TGAAAACAAT GAAAATGGTC ACGGTAATTA CCACGTGGAT ATTAAGAGTG GTAATCAGTC ATTTGAGGAA CAAGGTGGCA ATATCGTAGA TGGTCAAAAT AATGGTAACC AACCTAAGTA TGAACAAGGT GTGCCACATA TTCACGGATT TACAAATAAA GATAAACC AA ACTTTGAAGA AGATACACTT CAAACGATTG AAGAAGATAC GACACCAGAA GTACCAAGCG AAGTACCAAG CGAGCCGGAA ACTGAACCTG GTAAACCAAT TTCTAAACCA GTGGAACAAG ATGAAAAGGT TAAAGCAGTG TCTGAACTAC CTGAAACAGG GTTCGGCGGA TTATTTAGCA AAAAGAATCA CAAAGCATAA AATTATATGA AGTAAGCCTG AATAATTACA TACTCAA TGA ATGACGTTGA TACATAGATT TGAAAAAAAT AAAAAAGGCA TTTTTACCAG CAACTAAAGC. ______________________________________
A plasmid or phage comprising a nucleotide sequence from S. aureus coding for a protein or polypeptide having fibronectin binding activity, said nucleotide sequence comprising:
- __________________________________________________________________________ GTTAACAACA ATCTTAACTT TTTATTAACT CGCTTTTTTT CATTGCTTTT AAAAACCGAA CAATATAGAA TTGCATTTAT TGAGTTTTTA AAATAAATGA ATTTTGCATT TAAGGGAGAA TATTATAGTG AAAAGCAATC TTAGATACGG CATAAGAAAA CACAAATTGG GAGCGGCCTC AGTATTCTTA GGAACAATGA TCGTTGTTGG AATGGGACAA GAAAAAGAAG CTGCAGCATC GGAACAAAAC AATACTACAG TAGAGGAAAG TGGGAGTTCA GCTACTGAAA GTAAAGCAAG CGAAACACAA ACAACTACAA ATAACGTTAA TACAATAGAT CAAACACAAT CATACAGCGC GACATCAACT GAGCAACCAT CACAATCAAC ACAAGTAACA ACAGAAGAAG CACCGAAAAC TGTGCAAGCA CCAAAAGTAG AAACTTCGCG AGTTGATTTG CCATCGGAAA AAGTTGCTGA TAAGGAAACT ACAGGAACTC AAGTTGACAT AGCTCAACAA AGTAAAGTCT CAGAAATTAA ACCAAGAATG AAAAGATCAA CTGACGTTAC AGCAGTTGCA GAGAAAGAAG TAGTGGAAGA AACTAAAGCG ACAGGTACAG ATGTAACAAA TAAAGTGGAA GTAGAAGAAG GTAGTGAAAT TGTAGGACAT AAACAAGATA CGAATGTTGT AAATCCTCAT AACGCAGAAA GAGTAACCTT GAAATATAAA TGGAAATTTG GAGAAGGAAT TAAGGCGGGA GATTATTTTG ATTTCACATT AAGCGATAAT GTTGAAACTC ATGGTATCTC AACACTGCGT AAAGTTCCGG AGATAAAAAG TACAGATGGT CAAGTTATGG CGACAGGAGA AATAATTGGA GAAAGAAAAG TTAGATATAC GTTTAAAGAA TATGTACAAG AAAAGAAAGA TTTAACTGCT GAATTATCTT TAAATCTATT TATTGATCCT ACAACAGTGA CGCAAAAAGG TAACCAAAAT GTTGAAGTTA AATTGGGTGA GACTACGGTT AGCAAAATAT TTAATATTCA ATATTTAGGT GGAGTTAGAG ATAATTGGGG AGTAACAGCT AATGGTCGAA TTGATACTTT AAATAAAGTA GATGGGAAAT TTAGTCATTT TGCGTACATG AAACCTAACA ACCAGTCGTT AAGCTCTGTG ACAGTAACTG GTCAAGTAAC TAAAGGAAAT AAACCAGGGG TTAATAATCC AACAGTTAAG GTATATAAAC ACATTGGTTC AGACGATTTA GCTGAAAGCG TATATGCAAA GCTTGATGAT GTCAGCAAAT TTGAAGATGT GACTGATAAT ATGAGTTTAG ATTTTGATAC TAATGGTGGT TATTCTTTAA ACTTTAATAA TTTAGACCAA AGTAAAAATT ATGTAATAAA ATATGAAGGG TATTATGATT CAAATGCTAG CAACTTAGAA TTTCAAACAC ACCTTTTTGG ATATTATAAC TATTATTATA CAAGTAATTT AACTTGGAAA AATGGCGTTG CATTTTACTC TAATAACGCT CAAGGCGACG GCAAAGATAA ACTAAAGGAA CCTATTATAG AACATAGTAC TCCTATCGAA CTTGAATTTA AATCAGAGCC GCCAGTGGAG AAGCATGAAT TGACTGGTAC AATCGAAGAA AGTAATGATT CTAAGCCAAT TGATTTTGAA TATCATACAG CTGTTGAAGG TGCAGAAGGT CATGCAGAAG GTACCATTGA AACTGAAGAA GATTCTATTC ATGTAGACTT TGAAGAATCG ACACATGAAA ATTCAAAACA TCATGCTGAT GTTGTTGAAT ATGAAGAAGA TACAAACCCA GGTGGTGGTC AGGTTACTAC TGAGTCTAAC CTAGTTGAAT TTGACGAAGA TTCTACAAAA GGTATTGTAA CTGGTGCTGT TAGCGATCAT ACAACAATTG AAGATACGAA AGAATATACG ACTGAAAGTA ACTTGATTGA ACTAGTAGAT GAACTACCTG AAGAACATGG TCAAGCGCAA GGACCAATCG AGGAAATTAC TGAAAACAAT CATCATATTT CTCATTCTGG TTTAGGAACT GAAAATGGTC ACGGTAATTA TGGCGTGATT GAAGAAATCG AAGAAAATAG CCACGTGGAT ATTAAGAGTG AATTAGGTTA CGAAGGTGGC CAAAATAGCG GTAATCAGTC ATTTGAGGAA GACACAGAAG AAGATAAACC GAAATATGAA CAAGGTGGCA ATATCGTAGA TATCGATTTC GATAGTGTAC CTCAAATTCA TGGTCAAAAT AATGGTAACC AATCATTCGA AGAAGATACA GAGAAAGACA AACCTAAGTA TGAACAAGGT GGTAATATCA TTGATATCGA CTTCGACAGT GTGCCACATA TTCACGGATT CAATAAGCAC ACTGAAATTA TTGAAGAAGA TACAAATAAA GATAAACCAA ATTATCAATT CGGTGGACAC AATAGTGTTG ACTTTGAAGA AGATACACTT CCACAAGTAA GTGGTCATAA TGAAGGTCAA CAAACGATTG AAGAAGATAC AACACCTCCA ATCGTGCCAC CAACGCCACC GACACCAGAA GTACCAAGCG AGCCGGAAAC ACCAACACCA CCGACACCAG AAGTACCAAG CGAGCCGGAA ACACCAACAC CGCCAACGCC AGAGGTACCA ACTGAACCTG GTAAACCAAT ACCACCTGCT AAAGAAGAAC CTAAAAAACC TTCTAAACCA GTGGAACAAG GTAAAGTAGT AACACCTGTT ATTGAAATCA ATGAAAAGGT TAAAGCAGTG GTACCAACTA AAAAAGCACA ATCTAAGAAA TCTGAACTAC CTGAAACAGG TGGAGAAGAA TCAACAAACA ACGGCATGTT GTTCGGCGGA TTATTTAGCA TTTTAGGTTT AGCGTTATTA CGCAGAAATA AAAAGAATCA CAAAGCATAA TCAATCCAAA ATTGACAGGT TTATTTCATA AATTATATGA AGTAAGCCTG TTTTTTAAAA TTAAAACAAA TTTCCCAAGA AATAATTACA TACTCAATGA CACTATGAAG GCGTTCTAAT TAGTGTTAAA ATGACGTTGA TACATAGATT TAATACTTAG GAAAAGGAGC ACATTAACTT TGAAAAAAAT AAAAAAGGCA ATCATTCCCG CTGCTGGTTT AGGGACTAGA TTTTTACCAG CAACTAAAGC TATCGATTTC GATAGTGTAC CTCAAATTCA TGGTCAAAAT AATGGTAACC AATCATTCGA AGAAGATACA GAGAAAGACA AACCTAAGTA TGAACAAGGT GGTAATATCA TTGATATCGA CTTCGACAGT GTGCCACATA TTCACGGATT CAATAAGCAC ACTGAAATTA TTGAAGAAGA TACAAATAAA GATAAACCAA ATTATCAATT CGGTGGACAC AATAGTGTTG ACTTTGAAGA AGATACACTT CCACAAGTAA GTGGTCATAA TGAAGGTCAA CAAACGATTG AAGAAGATAC AACACCTCCA ATCGTGCCAC CAACGCCACC GACACCAGAA GTACCAAGCG AGCCGGAAAC ACCAACACCA CCGACACCAG AAGTACCAAG CGAGCCGGAA ACACCAACAC CGCCAACGCC AGAGGTACCA ACTGAACCTG GTAAACCAAT ACCACCTGCT AAAGAAGAAC CTAAAAAACC TTCTAAACCA GTGGAACAAG GTAAAGTAGT AACACCTGTT ATTGAAATCA ATGAAAAGGT TAAAGCAGTG GTACCAACTA AAAAAGCACA ATCTAAGAAA TCTGAACTAC CTGAAACAGG TGGAGAAGAA TCAACAAACA ACGGCATGTT GTTCGGCGGA TTATTTAGCA TTTTAGGTTT AGCGTTATTA CGCAGAAATA AAAAGAATCA CAAAGCATAA TCAATCCAAA ATTGACAGGT TTATTTCATA AATTATATGA AGTAAGCCTG TTTTTTAAAA TTAAAACAAA TTTCCCAAGA AATAATTACA TACTCAATGA CACTATGAAG GCGTTCTAAT TAGTGTTAAA ATGACGTTGA TACATAGATT TAATACTTAG GAAAAGGAGC ACATTAACTT TGAAAAAAAT AAAAAAGGCA ATCATTCCCG CTGCTGGTTT AGGGACTAGA TTTTTACCAG CAACTAAAGC. __________________________________________________________________________
An E. coli strain expressing a fibronectin binding protein encoded by the nucleotide sequence:
- __________________________________________________________________________ GTTAACAACA ATCTTAACTT TTTATTAACT CGCTTTTTTT CATTGCTTTT AAAAACCGAA CAATATAGAA TTGCATTTAT TGAGTTTTTA AAATAAATGA ATTTTGCATT TAAGGGAGAA TATTATAGTG AAAAGCAATC TTAGATACGG CATAAGAAAA CACAAATTGG GAGCGGCCTC AGTATTCTTA GGAACAATGA TCGTTGTTGG AATGGGACAA GAAAAAGAAG CTGCAGCATC GGAACAAAAC AATACTACAG TAGAGGAAAG TGGGAGTTCA GCTACTGAAA GTAAAGCAAG CGAAACACAA ACAACTACAA ATAACGTTAA TACAATAGAT GAAACACAAT CATACAGCGC GACATCAACT GAGCAACCAT CACAATCAAC ACAAGTAACA ACAGAAGAAG CACCGAAAAC TGTGCAAGCA CCAAAAGTAG AAACTTCGCG AGTTGATTTG CCATCGGAAA AAGTTGCTGA TAAGGAAACT ACAGGAACTC AAGTTGACAT AGCTCAACAA AGTAAAGTCT CAGAAATTAA ACCAAGAATG AAAAGATCAA CTGACGTTAC AGCAGTTGCA GAGAAAGAAG TAGTGGAAGA AACTAAAGCG ACAGGTACAG ATGTAACAAA TAAAGTGGAA GTAGAAGAAG GTAGTGAAAT TGTAGGACAT AAACAAGATA CGAATGTTGT AAATCCTCAT AACGCAGAAA GAGTAACCTT GAAATATAAA TGGAAATTTG GAGAAGGAAT TAAGGCGGGA GATTATTTTG ATTTCACATT AAGCGATAAT GTTGAAACTC ATGGTATCTC AACACTGCGT AAAGTTCCGG AGATAAAAAG TACAGATGGT CAAGTTATGG CGACAGGAGA AATAATTGGA GAAAGAAAAG TTAGATATAC GTTTAAAGAA TATGTACAAG AAAAGAAAGA TTTAACTGCT GAATTATCTT TAAATCTATT TATTGATCCT ACAACAGTGA CGCAAAAAGG TAACCAAAAT GTTGAAGTTA AATTGGGTGA GACTACGGTT AGCAAAATAT TTAATATTCA ATATTTAGGT GGAGTTAGAG ATAATTGGGG AGTAACAGCT AATGGTCGAA TTGATACTTT AAATAAAGTA GATGGGAAAT TTAGTCATTT TGCGTACATG AAACCTAACA ACCAGTCGTT AAGCTCTGTG ACAGTAACTG GTCAAGTAAC TAAAGGAAAT AAACCAGGGG TTAATAATCC AACAGTTAAG GTATATAAAC ACATTGGTTC AGACGATTTA GCTGAAAGCG TATATGCAAA GCTTGATGAT GTCAGCAAAT TTGAAGATGT GACTGATAAT ATGAGTTTAG ATTTTGATAC TAATGGTGGT TATTCTTTAA ACTTTAATAA TTTAGACCAA AGTAAAAATT ATGTAATAAA ATATGAAGGG TATTATGATT CAAATGCTAG CAACTTAGAA TTTCAAACAC ACCTTTTTGG ATATTATAAC TATTATTATA CAAGTAATTT AACTTGGAAA AATGGCGTTG CATTTTACTC TAATAACGCT CAAGGCGACG GCAAAGATAA ACTAAAGGAA CCTATTATAG AACATAGTAC TCCTATCGAA CTTGAATTTA AATCAGAGCC GCCAGTGGAG AAGCATGAAT TGACTGGTAC AATCGAAGAA AGTAATGATT CTAAGCCAAT TGATTTTGAA TATCATACAG CTGTTGAAGG TGCAGAAGGT CATGCAGAAG GTACCATTGA AACTGAAGAA GATTCTATTC ATGTAGACTT TGAAGAATCG ACACATGAAA ATTCAAAACA TCATGCTGAT GTTGTTGAAT ATGAAGAAGA TACAAACCCA GGTGGTGGTC AGGTTACTAC TGAGTCTAAC CTAGTTGAAT TTGACGAAGA TTCTACAAAA GGTATTGTAA CTGGTGCTGT TAGCGATCAT ACAACAATTG AAGATACGAA AGAATATACG ACTGAAAGTA ACTTGATTGA ACTAGTAGAT GAACTACCTG AAGAACATGG TCAAGCGCAA GGACCAATCG AGGAAATTAC TGAAAACAAT CATCATATTT CTCATTCTGG TTTAGGAACT GAAAATGGTC ACGGTAATTA TGGCGTGATT GAAGAAATCG AAGAAAATAG CCACGTGGAT ATTAAGAGTG AATTAGGTTA CGAAGGTGGC CAAAATAGCG GTAATCAGTC ATTTGAGGAA GACACAGAAG AAGATAAACC GAAATATGAA CAAGGTGGCA ATATCGTAGA TATCGATTTC GATAGTGTAC CTCAAATTCA TGGTCAAAAT AATGGTAACC AATCATTCGA AGAAGATACA GAGAAAGACA AACCTAAGTA TGAACAAGGT GGTAATATCA TTGATATCGA CTTCGACAGT GTGCCACATA TTCACGGATT CAATAAGCAC ACTGAAATTA TTGAAGAAGA TACAAATAAA GATAAACCAA ATTATCAATT CGGTGGACAC AATAGTGTTG ACTTTGAAGA AGATACACTT CCACAAGTAA GTGGTCATAA TGAAGGTCAA CAAACGATTG AAGAAGATAC AACACCTCCA ATCGTGCCAC CAACGCCACC GACACCAGAA GTACCAAGCG AGCCGGAAAC ACCAACACCA CCGACACCAG AAGTACCAAG CGAGCCGGAA ACACCAACAC CGCCAACGCC AGAGGTACCA ACTGAACCTG GTAAACCAAT ACCACCTGCT AAAGAAGAAC CTAAAAAACC TTCTAAACCA GTGGAACAAG GTAAAGTAGT AACACCTGTT ATTGAAATCA ATGAAAAGGT TAAAGCAGTG GTACCAACTA AAAAAGCACA ATCTAAGAAA TCTGAACTAC CTGAAACAGG TGGAGAAGAA TCAACAAACA ACGGCATGTT GTTCGGCGGA TTATTTAGCA TTTTAGGTTT AGCGTTATTA CGCAGAAATA AAAAGAATCA CAAAGCATAA TCAATCCAAA ATTGACAGGT TTATTTCATA AATTATATGA AGTAAGCCTG TTTTTTAAAA TTAAAACAAA TTTCCCAAGA AATAATTACA TACTCAATGA CACTATGAAG GCGTTCTAAT TAGTGTTAAA ATGACGTTGA TACATAGATT TAATACTTAG GAAAAGGAGC ACATTAACTT TGAAAAAAAT AAAAAAGGCA ATCATTCCCG CTGCTGGTTT AGGGACTAGA TTTTTACCAG CAACTAAAGC. __________________________________________________________________________
Dependencies are inferred from the claim text.