Rapid analysis of plural components

A method for analyzing glucose and mono-sodium glutamate in a liquid containing them, which comprises the steps:

• (a) adding a volume of the liquid and a pH buffer solution to a reaction cell designed to prevent air from entering the cell, said reaction cell being equipped with an oxygen sensor connected to an automatic recording device,
• (b) adding an enzyme selected from the group consisting of glucose oxidase and glutamic acid oxidase to the liquid which catalyzes oxidation of one component in the liquid,
• (c) continuously recording oxygen sensor output,
• (d) adding an enzyme selected from the group consisting of glucose oxidase and glutamic acid oxidase which is different from the enzyme added in step (b) to the liquid which catalyzes oxidation of a second component in the liquid,
• (e) continuously recording oxygen sensor output, and
• (f) calculating the amount of said one component of step (b) from the decrease in oxygen sensor output in step (c) and calculating the amount of said second component present in the liquid from the decrease in oxygen sensor output in step (e).

A method for analyzing inosinic acid and glutamic acid in a liquid containing them, which comprises the steps:

• (a) adding a volume of the liquid and a pH buffer solution to a reaction cell designed to prevent air from entering the cell, said reaction cell being equipped with an oxygen sensor connected to an automatic recording device,
• (b) adding xanthene oxidase and an enzyme selected from the group consisting of nucleoside phosphorylase and glutamic acid oxidase to the liquid,
• (c) continuously recording oxygen sensor output,
• (d) adding an enzyme selected from the group consisting of nucleoside phosphorylase and glutamic acid oxidase which is different from the enzyme added in step (b) to the liquid which catalyzes oxidation of a second component in the liquid,
• (e) continuously recording oxygen sensor output, and
• (f) calculating the amount of said one component of step (b) from the decrease in oxygen sensor output in step (c) and calculating the amount of said second component present in the liquid from the decrease in oxygen sensor output in step (e) provided that when nucleoside phosphorylase is the added enzyme alkaline phosphatase is added together with the nucleoside phosphorylase.

A method for analyzing glutamic acid and glucose in a liquid containing them, which comprises the steps:

• (a) adding a volume of the liquid and a pH buffer solution to a reaction cell designed to prevent air from entering the cell, said reaction cell being equipped with an oxygen sensor connected to an automatic recording device,
• (b) adding an enzyme selected from the group consisting of glutamic acid oxidase and glucose oxidase to the liquid which catalyzes oxidation of one component in the liquid,
• (c) continuously recording oxygen sensor output,
• (d) adding an enzyme selected from the group consisting of glutamic acid oxidase and glucose oxidase which is different from the enzyme added in step (b) to the liquid which catalyzes oxidation of a second component in the liquid,
• (e) continuously recording oxygen sensor output, and
• (f) calculating the amount of said one component of step (b) from the decrease in oxygen sensor output in step (c) and calculating the amount of said second component present in the liquid from the decrease in oxygen sensor output in step (e).

A method for analyzing ascorbic acid and glucose in a liquid containing them, which comprises the steps:

• (a) adding a volume of the liquid and a pH buffer solution to a reaction cell designed to prevent air from entering the cell, said reaction cell being equipped with an oxygen sensor connected to an automatic recording device,
• (b) adding an enzyme selected from the group consisting of ascorbate oxidase and glucose oxidase to the liquid which catalyzes oxidation of one component in the liquid,
• (c) continuously recording oxygen sensor output,
• (d) adding an enzyme selected from the group consisting of ascorbate oxidase and glucose oxidase which is different from the enzyme added in step (b) to the liquid which catalyzes oxidation of a second component in the liquid,
• (e) continuously recording oxygen sensor output, and
• (f) calculating the amount of said one component of step (b) from the decrease in oxygen sensor output in step (c) and calculating the amount of said second component present in the liquid from the decrease in oxygen sensor output in step (e).