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US 5,175,082 AGranted
Method of characterizing genomic DNA
AssigneeImperial Chemical Industries PLC
InventorAlec J. Jeffreys
Life of this patent
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Abstract
Test samples of genomic DNA may be characterized by the use of polynucleotide probes each of which is specific for an informative genetic locus. Such probes may be prepared by the use of probes which are capable of differentiating DNA by reference to more than one polymorphic minisatellite region or hypervariable locus. The polynucleotides and probes of the invention are of use for genetic identification purposes, paternity and maternity testing and particularly in forensic medicine.
Claims8
A method of characterizing a test sample of genomic DNA from a donor by reference to one or more controls which method comprises:
- i) fragmenting said sample DNA with at least one restriction enzyme which does not cleave a sequence corresponding to a tandem repeat,
- ii) probing the DNA fragments with a polynucleotide probe which specifically hybridizes to a single minisatellite region (to the exclusion of other regions),
- wherein said probe consists essentially of up to 10,000 tandem repeats of a repeat sequence unit being one of ##STR7## wherein Y is C, T or U, X is G or C, R is A or G and T is T or U, or a sequence complementary thereto of identical length,
- iii) detecting hybridized fragments of DNA, and
- iv) comparing the hybridized fragments with the said control or controls.
2 more elements
Dependencies are inferred from the claim text.