Process for the preparation of dispersible colloidal systems of amphiphilic lipids in the form of oligolamellar liposomes of submicron dimensions

A process for the preparation of dispersible colloidal systems of amphiphilic lipids in the form of oligolamellar liposomes of submicron dimensions, the wall of which is constituted by said lipids and the interior of which is constituted by water or by an aqueous solution, comprising:

• combining (1) a first liquid phase consisting essentially of a solution of said lipids in a volatile organic solvent for said lipids or in a mixture of volatile organic solvents for said lipids, and (2) a greater amount of a second liquid phase consisting essentially of water, by pouring one of the phases into the other, so as substantially instantaneously to form a colloidal suspension of oligolamellar liposomes having a size ranging from about 100 to about 300 nm and comprising a core of said second phase surrounded by a layer of said lipids, said solvent or mixture of solvents of the first phase being miscible in all proportions with water, said process being performed in the absence of elevated temperature.